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Literature DB >> 12151334

Murine spermatogonial stem cells: targeted transgene expression and purification in an active state.

Galicia Giuili¹, Andrea Tomljenovic, Nathalie Labrecque, Mustapha Oulad-Abdelghani, Minoo Rassoulzadegan, François Cuzin.

Abstract

A 400 bp fragment of the spermatogonia-specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP-Cam, and the beta1- and alpha6-integrins characteristic of the stem cell fraction. A 700-fold enrichment in stem cells was determined by the ability of the purified fractions to re-establish spermatogenesis in germ cell-depleted recipient testes.

Entities: Gene Species

Mesh：

Substances：

Year: 2002 PMID： 12151334 PMCID： PMC1084203 DOI： 10.1093/embo-reports/kvf149

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

19 in total

1. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells.

Authors: T Shinohara; K E Orwig; M R Avarbock; R L Brinster
Journal: Proc Natl Acad Sci U S A Date: 2000-07-18 Impact factor: 11.205

2. From hematopoiesis to neuropoiesis: evidence of overlapping genetic programs.

Authors: A V Terskikh; M C Easterday; L Li; L Hood; H I Kornblum; D H Geschwind; I L Weissman
Journal: Proc Natl Acad Sci U S A Date: 2001-07-03 Impact factor: 11.205

3. A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection.

Authors: D J Elliott; C F Bourgeois; A Klink; J Stévenin; H J Cooke
Journal: Proc Natl Acad Sci U S A Date: 2000-05-23 Impact factor: 11.205

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

5. Transplantation of male germ line stem cells restores fertility in infertile mice.

Authors: T Ogawa; I Dobrinski; M R Avarbock; R L Brinster
Journal: Nat Med Date: 2000-01 Impact factor: 53.440

6. Transgenic mice produced by retroviral transduction of male germ-line stem cells.

Authors: M Nagano; C J Brinster; K E Orwig; B Y Ryu; M R Avarbock; R L Brinster
Journal: Proc Natl Acad Sci U S A Date: 2001-10-23 Impact factor: 11.205

7. Spermatogenesis following male germ-cell transplantation.

Authors: R L Brinster; J W Zimmermann
Journal: Proc Natl Acad Sci U S A Date: 1994-11-22 Impact factor: 11.205

8. Transactivation of the 'promoterless' luciferase-encoding vectors pXP1 and pXP2 by C/EBP alpha.

Authors: Y C Li; S Hayes; A P Young
Journal: Gene Date: 1994-01-28 Impact factor: 3.688

9. Gene trap analysis of germ cell signaling to Sertoli cells: NGF-TrkA mediated induction of Fra1 and Fos by post-meiotic germ cells.

Authors: F Vidal; P Lopez; L A López-Fernández; F Ranc; J C Scimeca; F Cuzin; M Rassoulzadegan
Journal: J Cell Sci Date: 2001-01 Impact factor: 5.285

10. Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene.

Authors: M Oulad-Abdelghani; P Bouillet; D Décimo; A Gansmuller; S Heyberger; P Dollé; S Bronner; Y Lutz; P Chambon
Journal: J Cell Biol Date: 1996-10 Impact factor: 10.539

39 in total

1. Interactions of laminin β3 fragment with β1-integrin receptor: A revisit of the apical ectoplasmic specialization-blood-testis-barrier-hemidesmosome functional axis in the testis.

Authors: C Yan Cheng; Pearl Py Lie; Ka-Wai Mok; Yan-Ho Cheng; Elissa Wp Wong; Jayakanthan Mannu; Premendu P Mathur; Helen H N Yan; Dolores D Mruk
Journal: Spermatogenesis Date: 2011-07-01

Murine spermatogonial stem cells: targeted transgene expression and purification in an active state.

1. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells.

2. From hematopoiesis to neuropoiesis: evidence of overlapping genetic programs.

3. A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection.

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

5. Transplantation of male germ line stem cells restores fertility in infertile mice.

6. Transgenic mice produced by retroviral transduction of male germ-line stem cells.

7. Spermatogenesis following male germ-cell transplantation.

8. Transactivation of the 'promoterless' luciferase-encoding vectors pXP1 and pXP2 by C/EBP alpha.

9. Gene trap analysis of germ cell signaling to Sertoli cells: NGF-TrkA mediated induction of Fra1 and Fos by post-meiotic germ cells.

10. Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene.

1. Interactions of laminin β3 fragment with β1-integrin receptor: A revisit of the apical ectoplasmic specialization-blood-testis-barrier-hemidesmosome functional axis in the testis.

Review 2. Signaling molecules and pathways regulating the fate of spermatogonial stem cells.

3. Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation.

4. Sex-specific timing of meiotic initiation is regulated by Cyp26b1 independent of retinoic acid signalling.

5. Epigenetic status determines germ cell meiotic commitment in embryonic and postnatal mammalian gonads.

6. Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice.

7. Isolation and cultivation of stem cells from adult mouse testes.

Review 8. Spermatogonial stem cell regulation and spermatogenesis.

9. Recent advances in application of male germ cell transplantation in farm animals.

10. c-Flip overexpression affects satellite cell proliferation and promotes skeletal muscle aging.