Literature DB >> 12148987

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.

Jason W Chin1, Stephen W Santoro, Andrew B Martin, David S King, Lei Wang, Peter G Schultz.   

Abstract

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

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Year:  2002        PMID: 12148987     DOI: 10.1021/ja027007w

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  210 in total

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2.  Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.

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4.  Surface-Mediated Protein Unfolding as a Search Process for Denaturing Sites.

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Journal:  ACS Nano       Date:  2015-11-25       Impact factor: 15.881

5.  Addicting diverse bacteria to a noncanonical amino acid.

Authors:  Drew S Tack; Jared W Ellefson; Ross Thyer; Bo Wang; Jimmy Gollihar; Matthew T Forster; Andrew D Ellington
Journal:  Nat Chem Biol       Date:  2016-01-18       Impact factor: 15.040

6.  Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation.

Authors:  Anirban Chakraborty; Abhishek Mazumder; Miaoxin Lin; Adam Hasemeyer; Qumiao Xu; Dongye Wang; Yon W Ebright; Richard H Ebright
Journal:  Methods Mol Biol       Date:  2015

7.  An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.

Authors:  Douglas D Young; Travis S Young; Michael Jahnz; Insha Ahmad; Glen Spraggon; Peter G Schultz
Journal:  Biochemistry       Date:  2011-02-01       Impact factor: 3.162

Review 8.  Incorporation of Non-Canonical Amino Acids.

Authors:  Lilia Leisle; Francis Valiyaveetil; Ryan A Mehl; Christopher A Ahern
Journal:  Adv Exp Med Biol       Date:  2015       Impact factor: 2.622

9.  Rapid discovery and evolution of orthogonal aminoacyl-tRNA synthetase-tRNA pairs.

Authors:  Daniele Cervettini; Shan Tang; Stephen D Fried; Julian C W Willis; Louise F H Funke; Lucy J Colwell; Jason W Chin
Journal:  Nat Biotechnol       Date:  2020-04-13       Impact factor: 54.908

10.  Resurrecting the Bacterial Tyrosyl-tRNA Synthetase/tRNA Pair for Expanding the Genetic Code of Both E. coli and Eukaryotes.

Authors:  James S Italia; Christopher Latour; Chester J J Wrobel; Abhishek Chatterjee
Journal:  Cell Chem Biol       Date:  2018-08-02       Impact factor: 8.116

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