BACKGROUND: Although there is a lot information in the literature about genome size in fish, a high variability among data for the same species is reported, being mainly related to methodological aspects. Flow cytometry-based fluorescence measurements of intercalating dyes is the most attractive approach due to its precision, objectivity, high speed, and relative simplicity. METHODS: We analyze the DNA content of G0/G1 diploid nuclei of three teleost species (Carassius auratus, Tinca tinca, and Danio rerio) using flow cytometry. Forty-three animals were used and up to 50,000 retinal cells were analyzed per sample. Propidium iodide-associated fluorescence was assessed using a FACSCalibur flow cytometer. Standard human leukocytes were used as a reference. RESULTS: Our results show that C. auratus (3.584 +/- 0.058 pg per nucleus) and D. rerio (3.357 +/- 0.074 pg per nucleus) showed similar DNA contents per cell, whereas it was significantly lower (2.398 +/- 0.038 pg per nucleus) in T. tinca. Interestingly, a low intraspecies variability was observed, the coefficient of variation being 1.608%, 2.198%, and 1.573% for C. auratus, D. rerio, and T. tinca, respectively. CONCLUSIONS: The methodology used in this study provides an accurate and easy measurement of the genome size of a species. Copyright 2002 Wiley-Liss, Inc.
BACKGROUND: Although there is a lot information in the literature about genome size in fish, a high variability among data for the same species is reported, being mainly related to methodological aspects. Flow cytometry-based fluorescence measurements of intercalating dyes is the most attractive approach due to its precision, objectivity, high speed, and relative simplicity. METHODS: We analyze the DNA content of G0/G1 diploid nuclei of three teleost species (Carassius auratus, Tinca tinca, and Danio rerio) using flow cytometry. Forty-three animals were used and up to 50,000 retinal cells were analyzed per sample. Propidium iodide-associated fluorescence was assessed using a FACSCalibur flow cytometer. Standard human leukocytes were used as a reference. RESULTS: Our results show that C. auratus (3.584 +/- 0.058 pg per nucleus) and D. rerio (3.357 +/- 0.074 pg per nucleus) showed similar DNA contents per cell, whereas it was significantly lower (2.398 +/- 0.038 pg per nucleus) in T. tinca. Interestingly, a low intraspecies variability was observed, the coefficient of variation being 1.608%, 2.198%, and 1.573% for C. auratus, D. rerio, and T. tinca, respectively. CONCLUSIONS: The methodology used in this study provides an accurate and easy measurement of the genome size of a species. Copyright 2002 Wiley-Liss, Inc.
Authors: Michael R Hall; Kevin M Kocot; Kenneth W Baughman; Selene L Fernandez-Valverde; Marie E A Gauthier; William L Hatleberg; Arunkumar Krishnan; Carmel McDougall; Cherie A Motti; Eiichi Shoguchi; Tianfang Wang; Xueyan Xiang; Min Zhao; Utpal Bose; Chuya Shinzato; Kanako Hisata; Manabu Fujie; Miyuki Kanda; Scott F Cummins; Noriyuki Satoh; Sandie M Degnan; Bernard M Degnan Journal: Nature Date: 2017-04-05 Impact factor: 49.962
Authors: Pedro L P Xavier; José A Senhorini; Matheus Pereira-Santos; Takafumi Fujimoto; Eduardo Shimoda; Luciano A Silva; Silvio A Dos Santos; George S Yasui Journal: Front Genet Date: 2017-09-25 Impact factor: 4.599