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Literature DB >> 12097242

Methods for cultivation of luminal parasitic protists of clinical importance.

Abstract

Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 2002 PMID： 12097242 PMCID： PMC118080 DOI： 10.1128/CMR.15.3.329-341.2002

Source DB: PubMed Journal: Clin Microbiol Rev ISSN： 0893-8512 Impact factor: 26.132

57 in total

1. A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples.

Authors: B Walderich; L Müller; R Bracha; J Knobloch; G D Burchard
Journal: Parasitol Res Date: 1997 Impact factor: 2.289

2. Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate.

Authors: S W Tan; M Singh; K T Thong; L C Ho; K T Moe; X Q Chen; G C Ng; E H Yap
Journal: Parasitol Res Date: 1996 Impact factor: 2.289

3. Clonal growth in Petri dishes of Entamoeba histolytica.

Authors: D E Mueller; W A Petri
Journal: Trans R Soc Trop Med Hyg Date: 1995 Jan-Feb Impact factor: 2.184

4. Extensive genetic diversity in Blastocystis hominis.

Authors: C G Clark
Journal: Mol Biochem Parasitol Date: 1997-07 Impact factor: 1.759

5. Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia.

Authors: H D Luján; M R Mowatt; L G Byrd; T E Nash
Journal: Proc Natl Acad Sci U S A Date: 1996-07-23 Impact factor: 11.205

6. Evaluation of four enzyme immunoassays for the detection of Giardia lamblia antigen in stool specimens.

Authors: B Maraha; A G Buiting
Journal: Eur J Clin Microbiol Infect Dis Date: 2000-06 Impact factor: 3.267

7. Blastocystis hominis: A simplified, high-efficiency method for clonal growth on solid agar.

Authors: K S Tan; G C Ng; E Quek; J Howe; N P Ramachandran; E H Yap; M Singh
Journal: Exp Parasitol Date: 2000-09 Impact factor: 2.011

8. Diagnosis by faecal culture of Dientamoeba fragilis infections in Australian patients with diarrhoea.

Authors: N Sawangjaroen; R Luke; P Prociv
Journal: Trans R Soc Trop Med Hyg Date: 1993 Mar-Apr Impact factor: 2.184

9. YI-S, a casein-free medium for axenic cultivation of Entamoeba histolytica, related Entamoeba, Giardia intestinalis and Trichomonas vaginalis.

Authors: L S Diamond; C G Clark; C C Cunnick
Journal: J Eukaryot Microbiol Date: 1995 May-Jun Impact factor: 3.346

Review 10. Systematic review of diagnostic tests for vaginal trichomoniasis.

Authors: S R Patel; W Wiese; S C Patel; C Ohl; J C Byrd; C A Estrada
Journal: Infect Dis Obstet Gynecol Date: 2000

163 in total

1. A new-generation 5-nitroimidazole can induce highly metronidazole-resistant Giardia lamblia in vitro.

Authors: Linda A Dunn; Anita G Burgess; Kenia G Krauer; Lars Eckmann; Patrice Vanelle; Maxime D Crozet; Frances D Gillin; Peter Upcroft; Jacqueline A Upcroft
Journal: Int J Antimicrob Agents Date: 2010-04-24 Impact factor: 5.283

2. Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica.

Authors: Afzal Husain; Dan Sato; Ghulam Jeelani; Fumika Mi-ichi; Vahab Ali; Makoto Suematsu; Tomoyoshi Soga; Tomoyoshi Nozaki
Journal: J Biol Chem Date: 2010-10-05 Impact factor: 5.157

3. Susceptibility in vitro of clinically metronidazole-resistant Trichomonas vaginalis to nitazoxanide, toyocamycin, and 2-fluoro-2'-deoxyadenosine.

Authors: Janelle M Wright; Linda A Dunn; Zygmunt Kazimierczuk; Anita G Burgess; Kenia G Krauer; Peter Upcroft; Jacqueline A Upcroft
Journal: Parasitol Res Date: 2010-06-09 Impact factor: 2.289

Methods for cultivation of luminal parasitic protists of clinical importance.

1. A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples.

2. Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate.

3. Clonal growth in Petri dishes of Entamoeba histolytica.

4. Extensive genetic diversity in Blastocystis hominis.

5. Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia.

6. Evaluation of four enzyme immunoassays for the detection of Giardia lamblia antigen in stool specimens.

7. Blastocystis hominis: A simplified, high-efficiency method for clonal growth on solid agar.

8. Diagnosis by faecal culture of Dientamoeba fragilis infections in Australian patients with diarrhoea.

9. YI-S, a casein-free medium for axenic cultivation of Entamoeba histolytica, related Entamoeba, Giardia intestinalis and Trichomonas vaginalis.

Review 10. Systematic review of diagnostic tests for vaginal trichomoniasis.

1. A new-generation 5-nitroimidazole can induce highly metronidazole-resistant Giardia lamblia in vitro.

2. Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica.

3. Susceptibility in vitro of clinically metronidazole-resistant Trichomonas vaginalis to nitazoxanide, toyocamycin, and 2-fluoro-2'-deoxyadenosine.

4. Two novel serum-free media for the culture of Trichomonas vaginalis.

5. 5-Nitroimidazole drugs effective against metronidazole-resistant Trichomonas vaginalis and Giardia duodenalis.

6. In vitro effects of thiazolides on Giardia lamblia WB clone C6 cultured axenically and in coculture with Caco2 cells.

7. Ultrastructural and molecular characterization of Balantidium coli isolated in the Philippines.

Review 8. Current world status of Balantidium coli.

Review 9. Emerging from obscurity: biological, clinical, and diagnostic aspects of Dientamoeba fragilis.

10. Trichomonas vaginalis contact-dependent cytolysis of epithelial cells.