Literature DB >> 9272565

A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples.

B Walderich1, L Müller, R Bracha, J Knobloch, G D Burchard.   

Abstract

A new method for the purification of protozoan cysts from feces was established, allowing to isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 and a dot-blot test.

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Year:  1997        PMID： 9272565     DOI： 10.1007/s004360050326

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  4 in total

Review 1.  Methods for cultivation of luminal parasitic protists of clinical importance.

Authors:  C Graham Clark; Louis S Diamond
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

2.  Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar.

Authors:  Shweta Srivastava; Sudha Bhattacharya; Jaishree Paul
Journal:  Exp Parasitol       Date:  2005-04-01       Impact factor: 2.011

Review 3.  Pathogenesis of intestinal amebiasis: from molecules to disease.

Authors:  M Espinosa-Cantellano; A Martínez-Palomo
Journal:  Clin Microbiol Rev       Date:  2000-04       Impact factor: 26.132

4.  Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures.

Authors:  Milka Luna-Nácar; José Navarrete-Perea; Bárbara Moguel; Raúl J Bobes; Juan P Laclette; Julio C Carrero
Journal:  PLoS One       Date:  2016-05-26       Impact factor: 3.240
  4 in total

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