| Literature DB >> 12050395 |
Ja-Mun Chong1, Kazuya Sakuma, Makoto Sudo, Toshio Osawa, Etsuko Ohara, Hiroshi Uozaki, Junji Shibahara, Kenji Kuroiwa, Shin-Ichi Tominaga, Yoshitaka Hippo, Hiroyuki Aburatani, Nobuaki Funata, Masashi Fukayama.
Abstract
The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC.Entities:
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Year: 2002 PMID： 12050395 PMCID： PMC136266 DOI： 10.1128/jvi.76.13.6825-6831.2002
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103