Literature DB >> 12039787

Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays.

Narayanan Jothikumar1, Mansel W Griffiths.   

Abstract

A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.

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Year:  2002        PMID： 12039787      PMCID： PMC123919          DOI： 10.1128/AEM.68.6.3169-3171.2002

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7.

Authors:  N Y Fortin; A Mulchandani; W Chen
Journal:  Anal Biochem       Date:  2001-02-15       Impact factor: 3.365

2.  Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli O157 in bovine fecal samples.

Authors:  P A Chapman; A T Malo; C A Siddons; M Harkin
Journal:  Appl Environ Microbiol       Date:  1997-07       Impact factor: 4.792

3.  Detection of Escherichia coli O157:H7 by multiplex PCR.

Authors:  P M Fratamico; S K Sackitey; M Wiedmann; M Y Deng
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

4.  Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli.

Authors:  V K Sharma; E A Dean-Nystrom; T A Casey
Journal:  Mol Cell Probes       Date:  1999-08       Impact factor: 2.365

5.  Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk.

Authors:  J L McKillip; M Drake
Journal:  J Food Prot       Date:  2000-07       Impact factor: 2.077

6.  Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation.

Authors:  I R Grant; H J Ball; M T Rowe
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

7.  PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5' nuclease (TaqMan) assay.

Authors:  R D Oberst; M P Hays; L K Bohra; R K Phebus; C T Yamashiro; C Paszko-Kolva; S J Flood; J M Sargeant; J R Gillespie
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

8.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948
  8 in total
  23 in total

Review 1.  Detection and quantification of gene expression in environmental bacteriology.

Authors:  Freddie H Sharkey; Ibrahim M Banat; Roger Marchant
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

2.  Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples.

Authors:  James L Bono; James E Keen; Laura C Miller; James M Fox; Carol G Chitko-McKown; Michael P Heaton; William W Laegreid
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

3.  Bio-Conjugated CNT-Bridged 3D Porous Graphene Oxide Membrane for Highly Efficient Disinfection of Pathogenic Bacteria and Removal of Toxic Metals from Water.

Authors:  Bhanu Priya Viraka Nellore; Rajashekhar Kanchanapally; Francisco Pedraza; Sudarson Sekhar Sinha; Avijit Pramanik; Ashton T Hamme; Zikri Arslan; Dhiraj Sardar; Paresh Chandra Ray
Journal:  ACS Appl Mater Interfaces       Date:  2015-08-20       Impact factor: 9.229

4.  A sensitive multiplex, real-time PCR assay for prospective detection of Shiga toxin-producing Escherichia coli from stool samples reveals similar incidences but variable severities of non-O157 and O157 infections in northern California.

Authors:  Martina I Lefterova; Kathleen A Slater; Indre Budvytiene; Patricia A Dadone; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2013-07-10       Impact factor: 5.948

5.  Multiplexed bead-based mesofluidic system for detection of food-borne pathogenic bacteria.

Authors:  Sheng-Quan Jin; Bin-Cheng Yin; Bang-Ce Ye
Journal:  Appl Environ Microbiol       Date:  2009-08-28       Impact factor: 4.792

6.  Real-time PCR detection of Vibrio vulnificus in oysters: comparison of oligonucleotide primers and probes targeting vvhA.

Authors:  Gitika Panicker; Asim K Bej
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

Review 7.  Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics.

Authors:  Amira Souii; Manel Ben M'hadheb-Gharbi; Jawhar Gharbi
Journal:  Food Sci Biotechnol       Date:  2016-02-29       Impact factor: 2.391

8.  Real-time PCR assay for detection and enumeration of Dekkera bruxellensis in wine.

Authors:  Trevor G Phister; David A Mills
Journal:  Appl Environ Microbiol       Date:  2003-12       Impact factor: 4.792

9.  Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.

Authors:  Hiroshi Fukushima; Jun Kawase; Yoshiki Etoh; Kumiko Sugama; Shunshuke Yashiro; Natsuko Iida; Keiji Yamaguchi
Journal:  Int J Microbiol       Date:  2010-09-28

10.  Comprehensive and rapid real-time PCR analysis of 21 foodborne outbreaks.

Authors:  Hiroshi Fukushima; Kazunori Katsube; Yoshie Tsunomori; Ryoko Kishi; Junko Atsuta; Yuko Akiba
Journal:  Int J Microbiol       Date:  2009-06-24
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