Literature DB >> 15006817

Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples.

James L Bono1, James E Keen, Laura C Miller, James M Fox, Carol G Chitko-McKown, Michael P Heaton, William W Laegreid.   

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.

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Year:  2004        PMID： 15006817      PMCID： PMC368350          DOI： 10.1128/AEM.70.3.1855-1857.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  27 in total

1.  Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays.

Authors:  Narayanan Jothikumar; Mansel W Griffiths
Journal:  Appl Environ Microbiol       Date:  2002-06       Impact factor: 4.792

2.  Escherichia coli O157 infection associated with a petting zoo.

Authors:  A E Heuvelink; C van Heerwaarden; J T M Zwartkruis-Nahuis; R van Oosterom; K Edink; Y T H P van Duynhoven; E de Boer
Journal:  Epidemiol Infect       Date:  2002-10       Impact factor: 2.451

3.  Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7.

Authors:  P Feng; K A Lampel; H Karch; T S Whittam
Journal:  J Infect Dis       Date:  1998-06       Impact factor: 5.226

4.  Outbreak of Escherichia coli O157:H7 and Campylobacter among attendees of the Washington County Fair-New York, 1999.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  1999-09-17       Impact factor: 17.586

5.  Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR.

Authors:  Y Hu; Q Zhang; J C Meitzler
Journal:  J Appl Microbiol       Date:  1999-12       Impact factor: 3.772

Review 6.  Diarrheagenic Escherichia coli.

Authors:  J P Nataro; J B Kaper
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

7.  A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7.

Authors:  J Meng; S Zhao; M P Doyle; S E Mitchell; S Kresovich
Journal:  Lett Appl Microbiol       Date:  1997-03       Impact factor: 2.858

Review 8.  Application of rapid-cycle real-time polymerase chain reaction for the detection of microbial pathogens: the Mayo-Roche Rapid Anthrax Test.

Authors:  James R Uhl; Constance A Bell; Lynne M Sloan; Mark J Espy; Thomas F Smith; Jon E Rosenblatt; Franklin R Cockerill
Journal:  Mayo Clin Proc       Date:  2002-07       Impact factor: 7.616

9.  Multistate outbreak of Escherichia coli O157:H7 infections associated with eating ground beef--United States, June-July 2002.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2002-07-26       Impact factor: 17.586

Review 10.  Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections.

Authors:  J C Paton; A W Paton
Journal:  Clin Microbiol Rev       Date:  1998-07       Impact factor: 26.132
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  8 in total

1.  Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni.

Authors:  Delphine Rapp; John Waller; Gale Brightwell; Richard W Muirhead
Journal:  Appl Environ Microbiol       Date:  2009-12-28       Impact factor: 4.792

2.  Association of Escherichia coli O157:H7 tir polymorphisms with human infection.

Authors:  James L Bono; James E Keen; Michael L Clawson; Lisa M Durso; Michael P Heaton; William W Laegreid
Journal:  BMC Infect Dis       Date:  2007-08-24       Impact factor: 3.090

3.  Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans.

Authors:  James L Bono; Timothy P L Smith; James E Keen; Gregory P Harhay; Tara G McDaneld; Robert E Mandrell; Woo Kyung Jung; Thomas E Besser; Peter Gerner-Smidt; Martina Bielaszewska; Helge Karch; Michael L Clawson
Journal:  Mol Biol Evol       Date:  2012-02-21       Impact factor: 16.240

4.  Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms.

Authors:  Monalisha Nayak; Deepak Singh; Himanshu Singh; Rishi Kant; Ankur Gupta; Shashank Shekhar Pandey; Swarnasri Mandal; Gurunath Ramanathan; Shantanu Bhattacharya
Journal:  Sci Rep       Date:  2013-11-20       Impact factor: 4.379

Review 5.  A review on detection methods used for foodborne pathogens.

Authors:  B Priyanka; Rajashekhar K Patil; Sulatha Dwarakanath
Journal:  Indian J Med Res       Date:  2016-09       Impact factor: 2.375

6.  Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices.

Authors:  C Sethulekshmi; C Latha; C J Anu
Journal:  Vet World       Date:  2018-02-03

7.  An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection.

Authors:  Yushan Zhang; Chang-Qing Xu; Tianyi Guo; Lingcheng Hong
Journal:  Sci Rep       Date:  2018-12-13       Impact factor: 4.379

8.  Phylogenetic classification of Escherichia coli O157:H7 strains of human and bovine origin using a novel set of nucleotide polymorphisms.

Authors:  Michael L Clawson; James E Keen; Timothy P L Smith; Lisa M Durso; Tara G McDaneld; Robert E Mandrell; Margaret A Davis; James L Bono
Journal:  Genome Biol       Date:  2009-05-22       Impact factor: 13.583
  8 in total

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