BACKGROUND: A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. METHOD: Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 microm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. RESULTS: The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. CONCLUSION: Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.
BACKGROUND: A noninvasive technique to evaluate the structure and function of the kidney would be useful to investigate renal diseases, especially acute renal failure. We have developed a novel technique to visualize functional micro-magnetic resonance (MR) images of the mouse kidney with a dendrimer-based macromolecular renal MR contrast agent. METHOD:Mice were injected with cisplatin or vehicle, then examined three days later by contrast-enhanced, dynamic high-resolution micro-MRI with 160 microm spatial resolution using a 1.5 T clinical MRI unit, a surface coil, and the renal contrast agent G4D-(1B4M-Gd)64. RESULTS: The cortex and outer stripe of the outer medulla of the mouse kidney were clearly visualized in the normal mice. In animals treated with cisplatin, the gradation of tubular damage as assessed by contrast enhanced dynamic MRI correlated with renal function. CONCLUSION: Contrast-enhanced, dynamic high-resolution micro-MRI with a novel dendrimer-based macromolecular renal MR contrast agent can be a powerful tool for in vivo observation of renal structural and functional damage.
Authors: Lydia Würbach; Alexander Heidrich; Thomas Opfermann; Peter Gebhardt; Hans Peter Saluz Journal: Mol Imaging Biol Date: 2012-12 Impact factor: 3.488
Authors: Wojciech G Lesniak; Manoj K Mishra; Amar Jyoti; Bindu Balakrishnan; Fan Zhang; Elizabeth Nance; Roberto Romero; Sujatha Kannan; Rangaramanujam M Kannan Journal: Mol Pharm Date: 2013-10-30 Impact factor: 4.939
Authors: S Sadekar; O Linares; Gj Noh; D Hubbard; A Ray; M Janát-Amsbury; C M Peterson; J Facelli; H Ghandehari Journal: Drug Deliv Transl Res Date: 2013-06-01 Impact factor: 4.617
Authors: Jongdoo Lim; Baris Turkbey; Marcelino Bernardo; L Henry Bryant; Matteo Garzoni; Giovanni M Pavan; Takahito Nakajima; Peter L Choyke; Eric E Simanek; Hisataka Kobayashi Journal: Bioconjug Chem Date: 2012-10-22 Impact factor: 4.774