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Literature DB >> 1201008

Rapid separation of isolated hepatocytes or similar tissue fragments for analysis of cell constituents.

Abstract

A simple device is described for the rapid centrifugal separation of isolated hepatocytes or similar tissue fragments from the suspending medium. Rapid separation is essential if meaningful information on the concentrations of cell constituents and their distribution between cell and suspension medium is to be obtained. The usefulness of the technique is illustrated by measurements of hepatocyte constituents (glutamate, aspartate, alanine, K+) which show large concentration gradients against the extracellular medium.

Entities: Chemical

Mesh：

Substances：

Year: 1975 PMID： 1201008 PMCID： PMC1165702 DOI： 10.1042/bj1500047

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

5 in total

1. The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium.

Authors: W C WERKHEISER; W BARTLEY
Journal: Biochem J Date: 1957-05 Impact factor: 3.857

2. Concentrations of free glucogenic amino acids in livers of rats subjected to various metabolic stresses.

Authors: D H Williamson; O Lopes-Vieira; B Walker
Journal: Biochem J Date: 1967-08 Impact factor: 3.857

3. Transport of glutamine and glutamate in kidney mitochondria in relation to glutamine deamidation.

Authors: M Crompton; J B Chappell
Journal: Biochem J Date: 1973-01 Impact factor: 3.857

4. The effect of lysine on gluconeogenesis from lactate in rat hepatocytes.

Authors: N W Cornell; P Lund; H A Krebs
Journal: Biochem J Date: 1974-08 Impact factor: 3.857

5. Changes in the concentrations of hepatic metabolites on administration of dihydroxyacetone or glycerol to starved rats and their relationship to the control of ketogenesis.

Authors: D H Williamson; D Veloso; E V Ellington; H A Krebs
Journal: Biochem J Date: 1969-09 Impact factor: 3.857

5 in total

22 in total

1. Preparation and characterization of isolated parenchymal cells from guinea pig liver.

Authors: K R Elliott; C I Pogson
Journal: Mol Cell Biochem Date: 1977-05-31 Impact factor: 3.396

2. Microcarrier-attached rat hepatocytes as a xenobiotic-metabolizing system in cocultures.

Authors: J U Voss; H Seibert
Journal: Cell Biol Toxicol Date: 1991-10 Impact factor: 6.691

3. Gluconeogenesis in isolated intact lamb liver cells. Effects of glucagon and butyrate.

Authors: M G Clark; O H Filsell; I G Jarrett
Journal: Biochem J Date: 1976-06-15 Impact factor: 3.857

4. Inhibition of protein degradation in isolated rat hepatocytes.

Authors: M F Hopgood; M G Clark; F J Ballard
Journal: Biochem J Date: 1977-05-15 Impact factor: 3.857

5. Permeability of the liver cell membrane to quinolinate.

Authors: K R Elliott; C I Pogson; S A Smith
Journal: Biochem J Date: 1977-04-15 Impact factor: 3.857

6. Purine catabolism in isolated rat hepatocytes. Influence of coformycin.

Authors: G Van den Berghe; F Bontemps; H G Hers
Journal: Biochem J Date: 1980-06-15 Impact factor: 3.857

7. Glutamate, glutamine, aspartate, asparagine, glucose and ketone-body metabolism in chick intestinal brush-border cells.

Authors: J W Porteous
Journal: Biochem J Date: 1980-06-15 Impact factor: 3.857