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Literature DB >> 12010034

A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins.

Deepshikha Datta¹, Pin Wang, Isaac S Carrico, Stephen L Mayo, David A Tirrell.

Abstract

Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-Acetylphenylalanine was incorporated into recombinant dihydrofolate reductase (DHFR) in Escherichia coli via a computationally designed mutant form of the phenylalanyl-tRNA synthetase of the host. DHFR outfitted with ketone functionality can be chemoselectively ligated with hydrazide reagents under mild conditions.

Entities: Chemical Gene Species

Mesh：

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Year: 2002 PMID： 12010034 DOI： 10.1021/ja0177096

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

30 in total

1. Selectivity and specificity of substrate binding in methionyl-tRNA synthetase.

Authors: Deepshikha Datta; Nagarajan Vaidehi; Deqiang Zhang; William A Goddard
Journal: Protein Sci Date: 2004-10 Impact factor: 6.725

2. Second harmonic generating (SHG) nanoprobes for in vivo imaging.

Authors: Periklis Pantazis; James Maloney; David Wu; Scott E Fraser
Journal: Proc Natl Acad Sci U S A Date: 2010-07-28 Impact factor: 11.205

3. Click Grafting of Alkyne-containing Vinyl Polymers onto Biosynthesized Extracellular Matrix Protein Containing Azide Functionality and Adhesion Control of Human Umbilical Vein Endothelial Cells.

Authors: Tomoki Yamada; Akinori Takasu
Journal: RSC Adv Date: 2015-01-01 Impact factor: 3.361

4. Noncanonical amino acids in the interrogation of cellular protein synthesis.

Authors: John T Ngo; David A Tirrell
Journal: Acc Chem Res Date: 2011-08-04 Impact factor: 22.384

Review 5. Collagens as biomaterials.

Authors: John A M Ramshaw; Yong Y Peng; Veronica Glattauer; Jerome A Werkmeister
Journal: J Mater Sci Mater Med Date: 2008-04-01 Impact factor: 3.896

10. Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog.

Authors: Kenji Oki; Kensaku Sakamoto; Takatsugu Kobayashi; Hiroshi M Sasaki; Shigeyuki Yokoyama
Journal: Proc Natl Acad Sci U S A Date: 2008-09-02 Impact factor: 11.205

A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins.

1. Selectivity and specificity of substrate binding in methionyl-tRNA synthetase.

2. Second harmonic generating (SHG) nanoprobes for in vivo imaging.

3. Click Grafting of Alkyne-containing Vinyl Polymers onto Biosynthesized Extracellular Matrix Protein Containing Azide Functionality and Adhesion Control of Human Umbilical Vein Endothelial Cells.

4. Noncanonical amino acids in the interrogation of cellular protein synthesis.

Review 5. Collagens as biomaterials.

6. Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids.

Review 7. Click chemistry in complex mixtures: bioorthogonal bioconjugation.

8. Developing bioorthogonal probes to span a spectrum of reactivities.

Review 9. Multivalent protein polymers with controlled chemical and physical properties.

10. Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog.