Literature DB >> 15926061

Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR.

K Levi1, K J Towner.   

Abstract

The aim of this study was to evaluate real-time PCR (LightCycler) kits for the detection of methicillin-resistant Staphylococcus aureus in enrichment broth using 200 sets of patient screening swab samples. One hundred sets were processed using a lysostaphin/lysozyme extraction method and a further 100 were tested using a column extraction kit. The PCR kits with lysostaphin/lysozyme lysis showed 95.7% sensitivity, 90.8% specificity, and negative and positive predictive values of 98.6% and 75.9%, respectively, compared with 88%, 95.9%, 84.6% and 95.9%, respectively, with the column extraction kit.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 15926061     DOI： 10.1007/s10096-005-1336-4

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  15 in total

Review 1.  Would active surveillance cultures help control healthcare-related methicillin-resistant Staphylococcus aureus infections?

Authors:  Barry M Farr; William R Jarvis
Journal:  Infect Control Hosp Epidemiol       Date:  2002-02       Impact factor: 3.254

2.  Detection of methicillin/oxacillin resistance in staphylococci.

Authors:  D F Brown
Journal:  J Antimicrob Chemother       Date:  2001-07       Impact factor: 5.790

3.  Improved methods for detection of methicillin-resistant Staphylococcus aureus.

Authors:  S Rohrer; M Tschierske; R Zbinden; B Berger-Bächi
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2001-04       Impact factor: 3.267

4.  Preoperative screening of elective orthopaedic patients for MRSA.

Authors:  D El-Zimaity; S J Dawson; S Barrett; E Moseley
Journal:  J Hosp Infect       Date:  2004-02       Impact factor: 3.926

5.  Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997-1999.

Authors:  D J Diekema; M A Pfaller; F J Schmitz; J Smayevsky; J Bell; R N Jones; M Beach
Journal:  Clin Infect Dis       Date:  2001-05-15       Impact factor: 9.079

6.  Is it time to stop searching for MRSA? Screening is still important.

Authors:  B Cookson
Journal:  BMJ       Date:  1997-03-01

7.  Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay.

Authors:  Hong Fang; Göran Hedin
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

8.  Predictive value and cost-effectiveness analysis of a rapid polymerase chain reaction for preoperative detection of nasal carriage of Staphylococcus aureus.

Authors:  Nabin K Shrestha; Kenneth M Shermock; Steven M Gordon; Marion J Tuohy; Deborah A Wilson; Roberta E Cwynar; Michael K Banbury; David L Longworth; Carlos M Isada; Steven D Mawhorter; Gary W Procop
Journal:  Infect Control Hosp Epidemiol       Date:  2003-05       Impact factor: 3.254

9.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

10.  Cost-effectiveness of active surveillance cultures and contact/droplet precautions for control of methicillin-resistant Staphylococcus aureus.

Authors:  T B Karchmer; L J Durbin; B M Simonton; B M Farr
Journal:  J Hosp Infect       Date:  2002-06       Impact factor: 3.926
View more
  6 in total

Review 1.  Current trends in rapid diagnostics for methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococcus species.

Authors:  Surbhi Malhotra-Kumar; Kelly Haccuria; Mindy Michiels; Margareta Ieven; Claire Poyart; Waleria Hryniewicz; Herman Goossens
Journal:  J Clin Microbiol       Date:  2008-03-05       Impact factor: 5.948

Review 2.  Transmission rates, screening methods and costs of MRSA--a systematic literature review related to the prevalence in Germany.

Authors:  A Tübbicke; C Hübner; A Kramer; N-O Hübner; S Fleßa
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-05-10       Impact factor: 3.267

3.  Differentiation between Staphylococcus aureus and coagulase-negative Staphylococcus species by real-time PCR including detection of methicillin resistants in comparison to conventional microbiology testing.

Authors:  Sven Klaschik; Lutz E Lehmann; Folkert Steinhagen; Malte Book; Ernst Molitor; Andreas Hoeft; Frank Stueber
Journal:  J Clin Lab Anal       Date:  2014-05-05       Impact factor: 2.352

Review 4.  Evolving Rapid Methicillin-resistant Staphylococcus aureus Detection: Cover All the Bases.

Authors:  Yamuna Devi Bakthavatchalam; Laura E B Nabarro; Balaji Veeraraghavan
Journal:  J Glob Infect Dis       Date:  2017 Jan-Mar

5.  Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study.

Authors:  Stephan Harbarth; Cristina Masuet-Aumatell; Jacques Schrenzel; Patrice Francois; Christophe Akakpo; Gesuele Renzi; Jerome Pugin; Bara Ricou; Didier Pittet
Journal:  Crit Care       Date:  2006-02       Impact factor: 9.097

6.  PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.

Authors:  Ying Liu; Jiang Zhang; Yinduo Ji
Journal:  Open Microbiol J       Date:  2016-04-14
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.