Literature DB >> 11823428

Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex.

Thomas K Albert1, Hiroyuki Hanzawa, Yvonne I A Legtenberg, Marjolein J de Ruwe, Fiona A J van den Heuvel, Martine A Collart, Rolf Boelens, H Th Marc Timmers.   

Abstract

The RING finger protein CNOT4 is a component of the CCR4-NOT complex. This complex is implicated in repression of RNA polymerase II transcription. Here we demonstrate that CNOT4 functions as a ubiquitin-protein ligase (E3). We show that the unique C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes (E2s). Using NMR spectroscopy, we detail the interaction of CNOT4 with UbcH5B and characterize RING residues that are critical for this interaction. CNOT4 acts as a potent E3 ligase in vitro. Mutations that destabilize the E2-E3 interface abolish this activity. Based on these results, we present a model of how E3 ligase function within the CCR4-NOT complex relates to transcriptional regulation.

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Year:  2002        PMID： 11823428      PMCID： PMC125831          DOI： 10.1093/emboj/21.3.355

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  40 in total

1.  Solution structure of the N-terminal domain of the human TFIIH MAT1 subunit: new insights into the RING finger family.

Authors:  V Gervais; D Busso; E Wasielewski; A Poterszman; J M Egly; J C Thierry; B Kieffer
Journal:  J Biol Chem       Date:  2000-10-30       Impact factor: 5.157

2.  Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits.

Authors:  T K Albert; M Lemaire; N L van Berkum; R Gentz; M A Collart; H T Timmers
Journal:  Nucleic Acids Res       Date:  2000-02-01       Impact factor: 16.971

3.  Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p.

Authors:  E S Johnson; G Blobel
Journal:  J Biol Chem       Date:  1997-10-24       Impact factor: 5.157

Review 4.  The ubiquitin system.

Authors:  A Hershko; A Ciechanover
Journal:  Annu Rev Biochem       Date:  1998       Impact factor: 23.643

Review 5.  The RING finger domain: a recent example of a sequence-structure family.

Authors:  K L Borden; P S Freemont
Journal:  Curr Opin Struct Biol       Date:  1996-06       Impact factor: 6.809

6.  Crystal structure of the RAG1 dimerization domain reveals multiple zinc-binding motifs including a novel zinc binuclear cluster.

Authors:  S F Bellon; K K Rodgers; D G Schatz; J E Coleman; T A Steitz
Journal:  Nat Struct Biol       Date:  1997-07

7.  The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae.

Authors:  M Tucker; M A Valencia-Sanchez; R R Staples; J Chen; C L Denis; R Parker
Journal:  Cell       Date:  2001-02-09       Impact factor: 41.582

8.  TBP-associated factors are not generally required for transcriptional activation in yeast.

Authors:  Z Moqtaderi; Y Bai; D Poon; P A Weil; K Struhl
Journal:  Nature       Date:  1996-09-12       Impact factor: 49.962

9.  Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade.

Authors:  L Huang; E Kinnucan; G Wang; S Beaudenon; P M Howley; J M Huibregtse; N P Pavletich
Journal:  Science       Date:  1999-11-12       Impact factor: 47.728

10.  Interplay of positive and negative regulators in transcription initiation by RNA polymerase II holoenzyme.

Authors:  T I Lee; J J Wyrick; S S Koh; E G Jennings; E L Gadbois; R A Young
Journal:  Mol Cell Biol       Date:  1998-08       Impact factor: 4.272
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  90 in total

Review 1.  Ubiquitin and proteasomes in transcription.

Authors:  Fuqiang Geng; Sabine Wenzel; William P Tansey
Journal:  Annu Rev Biochem       Date:  2012-03-08       Impact factor: 23.643

2.  Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex.

Authors:  Peter S Brzovic; Jennifer R Keeffe; Hiroyuki Nishikawa; Keiko Miyamoto; David Fox; Mamoru Fukuda; Tomohiko Ohta; Rachel Klevit
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-05       Impact factor: 11.205

Review 3.  Getting into position: the catalytic mechanisms of protein ubiquitylation.

Authors:  Lori A Passmore; David Barford
Journal:  Biochem J       Date:  2004-05-01       Impact factor: 3.857

4.  Crystal structure of the human CNOT6L nuclease domain reveals strict poly(A) substrate specificity.

Authors:  Hui Wang; Masahiro Morita; Xiuna Yang; Toru Suzuki; Wen Yang; Jiao Wang; Kentaro Ito; Quan Wang; Cong Zhao; Mark Bartlam; Tadashi Yamamoto; Zihe Rao
Journal:  EMBO J       Date:  2010-07-13       Impact factor: 11.598

Review 5.  The structural basis for deadenylation by the CCR4-NOT complex.

Authors:  Mark Bartlam; Tadashi Yamamoto
Journal:  Protein Cell       Date:  2010-06-04       Impact factor: 14.870

6.  Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation.

Authors:  Claudia Temme; Lianbing Zhang; Elisabeth Kremmer; Christian Ihling; Aymeric Chartier; Andrea Sinz; Martine Simonelig; Elmar Wahle
Journal:  RNA       Date:  2010-05-26       Impact factor: 4.942

7.  De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay.

Authors:  Lisenka E L M Vissers; Sreehari Kalvakuri; Elke de Boer; Sinje Geuer; Machteld Oud; Inge van Outersterp; Michael Kwint; Melde Witmond; Simone Kersten; Daniel L Polla; Dilys Weijers; Amber Begtrup; Kirsty McWalter; Anna Ruiz; Elisabeth Gabau; Jenny E V Morton; Christopher Griffith; Karin Weiss; Candace Gamble; James Bartley; Hilary J Vernon; Kendra Brunet; Claudia Ruivenkamp; Sarina G Kant; Paul Kruszka; Austin Larson; Alexandra Afenjar; Thierry Billette de Villemeur; Kimberly Nugent; F Lucy Raymond; Hanka Venselaar; Florence Demurger; Claudia Soler-Alfonso; Dong Li; Elizabeth Bhoj; Ian Hayes; Nina Powell Hamilton; Ayesha Ahmad; Rachel Fisher; Myrthe van den Born; Marjolaine Willems; Arthur Sorlin; Julian Delanne; Sebastien Moutton; Philippe Christophe; Frederic Tran Mau-Them; Antonio Vitobello; Himanshu Goel; Lauren Massingham; Chanika Phornphutkul; Jennifer Schwab; Boris Keren; Perrine Charles; Maaike Vreeburg; Lenika De Simone; George Hoganson; Maria Iascone; Donatella Milani; Lucie Evenepoel; Nicole Revencu; D Isum Ward; Kaitlyn Burns; Ian Krantz; Sarah E Raible; Jill R Murrell; Kathleen Wood; Megan T Cho; Hans van Bokhoven; Maximilian Muenke; Tjitske Kleefstra; Rolf Bodmer; Arjan P M de Brouwer
Journal:  Am J Hum Genet       Date:  2020-06-17       Impact factor: 11.025

8.  The thick aleurone1 Gene Encodes a NOT1 Subunit of the CCR4-NOT Complex and Regulates Cell Patterning in Endosperm.

Authors:  Hao Wu; Bryan C Gontarek; Gibum Yi; Brandon D Beall; Anjanasree K Neelakandan; Bibechana Adhikari; Rumei Chen; Donald R McCarty; Andrew J Severin; Philip W Becraft
Journal:  Plant Physiol       Date:  2020-07-31       Impact factor: 8.340

9.  Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.

Authors:  Stephanie Pebernard; J Jefferson P Perry; John A Tainer; Michael N Boddy
Journal:  Mol Biol Cell       Date:  2008-07-30       Impact factor: 4.138

10.  Autographa californica multiple nucleopolyhedrovirus exon0 (orf141), which encodes a RING finger protein, is required for efficient production of budded virus.

Authors:  Xiaojiang Dai; Taryn M Stewart; Joseph A Pathakamuri; Qianjun Li; David A Theilmann
Journal:  J Virol       Date:  2004-09       Impact factor: 5.103
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