Literature DB >> 11812823

RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA).

B Verbeure1, E Lescrinier, J Wang, P Herdewijn.   

Abstract

Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.

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Year:  2001        PMID： 11812823      PMCID： PMC97599          DOI： 10.1093/nar/29.24.4941

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

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Review 2.  A critical survey of the structure-function of the antisense oligo/RNA heteroduplex as substrate for RNase H.

Authors:  E Zamaratski; P I Pradeepkumar; J Chattopadhyaya
Journal:  J Biochem Biophys Methods       Date:  2001-05-28

Review 3.  31P NMR of DNA.

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Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

4.  Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

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5.  Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering.

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Journal:  Biochem Biophys Res Commun       Date:  1983-12-16       Impact factor: 3.575

6.  Boranophosphates support the RNase H cleavage of polyribonucleotides.

Authors:  V K Rait; B R Shaw
Journal:  Antisense Nucleic Acid Drug Dev       Date:  1999-02

7.  Phosphorothioate oligonucleotides are inhibitors of human DNA polymerases and RNase H: implications for antisense technology.

Authors:  W Y Gao; F S Han; C Storm; W Egan; Y C Cheng
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8.  Assignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy.

Authors:  V Sklenár; H Miyashiro; G Zon; H T Miles; A Bax
Journal:  FEBS Lett       Date:  1986-11-10       Impact factor: 4.124

9.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

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Authors:  O Schatz; J Mous; S F Le Grice
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  15 in total

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Authors:  Jakob T Nielsen; Paul C Stein; Michael Petersen
Journal:  Nucleic Acids Res       Date:  2003-10-15       Impact factor: 16.971

2.  Synthesis and antisense properties of fluoro cyclohexenyl nucleic acid (F-CeNA), a nuclease stable mimic of 2'-fluoro RNA.

Authors:  Punit P Seth; Jinghua Yu; Ali Jazayeri; Pradeep S Pallan; Charles R Allerson; Michael E Østergaard; Fengwu Liu; Piet Herdewijn; Martin Egli; Eric E Swayze
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Review 3.  Non-natural nucleic acids for synthetic biology.

Authors:  Daniel H Appella
Journal:  Curr Opin Chem Biol       Date:  2009-10-29       Impact factor: 8.822

4.  Solution structure of the Dickerson DNA dodecamer containing a single ribonucleotide.

Authors:  Eugene F DeRose; Lalith Perera; Michael S Murray; Thomas A Kunkel; Robert E London
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5.  Design of antisense oligonucleotides stabilized by locked nucleic acids.

Authors:  Jens Kurreck; Eliza Wyszko; Clemens Gillen; Volker A Erdmann
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6.  Biochemical properties of phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides.

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7.  Oligonucleotides with cyclohexene-nucleoside building blocks: crystallization and preliminary X-ray studies of a left-handed sequence GTGTACAC.

Authors:  Koen Robeyns; Piet Herdewijn; Luc Van Meervelt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-06-01

8.  Antisense properties of tricyclo-DNA.

Authors:  Dorte Renneberg; Emilie Bouliong; Ulrich Reber; Daniel Schümperli; Christian J Leumann
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9.  Cyclohexenyl nucleic acids: conformationally flexible oligonucleotides.

Authors:  Koen Nauwelaerts; Eveline Lescrinier; Gert Sclep; Piet Herdewijn
Journal:  Nucleic Acids Res       Date:  2005-04-29       Impact factor: 16.971

10.  2'-fluoro-4'-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity.

Authors:  Jonathan K Watts; Niloufar Choubdar; Kashinath Sadalapure; Francis Robert; Alexander S Wahba; Jerry Pelletier; B Mario Pinto; Masad J Damha
Journal:  Nucleic Acids Res       Date:  2007-02-06       Impact factor: 16.971
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