G Yao1, G Chen, T Pan. 1. Department of Obstetrics and Gynecology, The Third Hospital, Peking University, Beijing, China. guimei2613@yahoo.com.cn
Abstract
PURPOSE: To determine the relationships between idiopathic oligo- or azoospermia and microdeletions of the Y chromosome. METHODS: Eighteen Y-linked sequence-tagged sites (STSs) in AZF (Azoospermia Factor) region were screened by means of multiplex PCR (Polymerase Chain Reaction) in 50 idiopathic infertile men, including 16 patients with azoospermia, 13 severe oligospermia, and 21 oligospermia. RESULTS: Microdeletions in the genomic DNA were observed in 8 of 50 cases, 3 with azoospermia, I severe oligospermia, and 4 oligospermia. Total deletion rate was 16.0% (8/50). The deletion regions were concentrated on AZFd and AZFc. CONCLUSIONS: Microdeletions of the Y chromosome are an important cause for idiopathic oligo- or azoospermia. Multiplex PCR is a useful technique for detecting the microdeletions. To avoid transmission to their offspring, patients with idiopathic oligo- or azoospermia should be screened for microdeletions of the Y chromosome before ICSI treatment for infertility.
PURPOSE: To determine the relationships between idiopathic oligo- or azoospermia and microdeletions of the Y chromosome. METHODS: Eighteen Y-linked sequence-tagged sites (STSs) in AZF (Azoospermia Factor) region were screened by means of multiplex PCR (Polymerase Chain Reaction) in 50 idiopathic infertile men, including 16 patients with azoospermia, 13 severe oligospermia, and 21 oligospermia. RESULTS: Microdeletions in the genomic DNA were observed in 8 of 50 cases, 3 with azoospermia, I severe oligospermia, and 4 oligospermia. Total deletion rate was 16.0% (8/50). The deletion regions were concentrated on AZFd and AZFc. CONCLUSIONS: Microdeletions of the Y chromosome are an important cause for idiopathic oligo- or azoospermia. Multiplex PCR is a useful technique for detecting the microdeletions. To avoid transmission to their offspring, patients with idiopathic oligo- or azoospermia should be screened for microdeletions of the Y chromosome before ICSI treatment for infertility.
Authors: M G Kent-First; S Kol; A Muallem; R Ofir; D Manor; S Blazer; N First; J Itskovitz-Eldor Journal: Mol Hum Reprod Date: 1996-12 Impact factor: 4.025
Authors: M Kent-First; A Muallem; J Shultz; J Pryor; K Roberts; W Nolten; L Meisner; A Chandley; G Gouchy; L Jorgensen; T Havighurst; J Grosch Journal: Mol Reprod Dev Date: 1999-05 Impact factor: 2.609
Authors: K Kobayashi; K Mizuno; A Hida; R Komaki; K Tomita; I Matsushita; M Namiki; T Iwamoto; S Tamura; S Minowada Journal: Hum Mol Genet Date: 1994-11 Impact factor: 6.150
Authors: R Oliva; E Margarit; J L Ballescá; A Carrió; A Sánchez; M Milà; L Jiménez; J R Alvarez-Vijande; F Ballesta Journal: Fertil Steril Date: 1998-09 Impact factor: 7.329
Authors: M A Coppola; K L Klotz; K-a Kim; H Y Cho; J Kang; J Shetty; S S Howards; C J Flickinger; J C Herr Journal: Hum Reprod Date: 2010-02-05 Impact factor: 6.918