Literature DB >> 11772607

Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

Pascale Serror1, Takashi Sasaki, S Dusko Ehrlich, Emmanuelle Maguin.   

Abstract

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per microg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii.

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Mesh：

Year:  2002        PMID： 11772607      PMCID： PMC126594          DOI： 10.1128/AEM.68.1.46-52.2002

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

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Authors:  M Fons; T Hégé; M Ladiré; P Raibaud; R Ducluzeau; E Maguin
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2.  High-molecular-weight linear multimer formation by single-stranded DNA plasmids in Escherichia coli.

Authors:  P Dabert; S D Ehrlich; A Gruss
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

3.  Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis.

Authors:  J J Godon; E Zumstein; P Dabert; F Habouzit; R Moletta
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4.  A target for carbon source-dependent negative regulation of the citB promoter of Bacillus subtilis.

Authors:  A Fouet; A L Sonenshein
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

5.  Transfection of Lactobacillus bulgaricus protoplasts by bacteriophage DNA.

Authors:  B Boizet; J L Flickinger; B M Chassy
Journal:  Appl Environ Microbiol       Date:  1988-12       Impact factor: 4.792

6.  Stable gene amplification in the chromosome of Bacillus subtilis.

Authors:  L Jannière; B Niaudet; E Pierre; S D Ehrlich
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Improved medium for lactic streptococci and their bacteriophages.

Authors:  B E Terzaghi; W E Sandine
Journal:  Appl Microbiol       Date:  1975-06

8.  Characterization of genetic elements required for site-specific integration of Lactobacillus delbrueckii subsp. bulgaricus bacteriophage mv4 and construction of an integration-proficient vector for Lactobacillus plantarum.

Authors:  L Dupont; B Boizet-Bonhoure; M Coddeville; F Auvray; P Ritzenthaler
Journal:  J Bacteriol       Date:  1995-02       Impact factor: 3.490

9.  Characterization, cloning, curing, and distribution in lactic acid bacteria of pLP1, a plasmid from Lactobacillus plantarum CCM 1904 and its use in shuttle vector construction.

Authors:  F Bringel; L Frey; J C Hubert
Journal:  Plasmid       Date:  1989-11       Impact factor: 3.466

10.  Characterization and sequence analysis of a small cryptic plasmid from Lactobacillus curvatus LTH683 and its use for construction of new Lactobacillus cloning vectors.

Authors:  J R Klein; C Ulrich; R Plapp
Journal:  Plasmid       Date:  1993-07       Impact factor: 3.466
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  20 in total

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Journal:  Clin Vaccine Immunol       Date:  2011-11-30

2.  The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution.

Authors:  M van de Guchte; S Penaud; C Grimaldi; V Barbe; K Bryson; P Nicolas; C Robert; S Oztas; S Mangenot; A Couloux; V Loux; R Dervyn; R Bossy; A Bolotin; J-M Batto; T Walunas; J-F Gibrat; P Bessières; J Weissenbach; S D Ehrlich; E Maguin
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-05       Impact factor: 11.205

3.  Comparative and functional analysis of sortase-dependent proteins in the predicted secretome of Lactobacillus salivarius UCC118.

Authors:  Jan-Peter van Pijkeren; Carlos Canchaya; Kieran A Ryan; Yin Li; Marcus J Claesson; Barbara Sheil; Lothar Steidler; Liam O'Mahony; Gerald F Fitzgerald; Douwe van Sinderen; Paul W O'Toole
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

4.  Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei.

Authors:  Hélène Licandro-Seraut; Sophie Brinster; Maarten van de Guchte; Hélène Scornec; Emmanuelle Maguin; Philippe Sansonetti; Jean-François Cavin; Pascale Serror
Journal:  Appl Environ Microbiol       Date:  2012-05-18       Impact factor: 4.792

5.  Production of a heterologous nonheme catalase by Lactobacillus casei: an efficient tool for removal of H2O2 and protection of Lactobacillus bulgaricus from oxidative stress in milk.

Authors:  Tatiana Rochat; Jean-Jacques Gratadoux; Alexandra Gruss; Gérard Corthier; Emmanuelle Maguin; Philippe Langella; Maarten van de Guchte
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

6.  Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins.

Authors:  Sigrid C J De Keersmaecker; Kristien Braeken; Tine L A Verhoeven; Mónica Perea Vélez; Sarah Lebeer; Jos Vanderleyden; Pascal Hols
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

7.  The insect peptide coprisin prevents Clostridium difficile-mediated acute inflammation and mucosal damage through selective antimicrobial activity.

Authors:  Jin Ku Kang; Jae Sam Hwang; Hyo Jung Nam; Keun Jae Ahn; Heon Seok; Sung-Kuk Kim; Eun Young Yun; Charalabos Pothoulakis; John Thomas Lamont; Ho Kim
Journal:  Antimicrob Agents Chemother       Date:  2011-08-01       Impact factor: 5.191

8.  Conjugative plasmid from Lactobacillus gasseri LA39 that carries genes for production of and immunity to the circular bacteriocin gassericin A.

Authors:  Yoshiyuki Ito; Yasushi Kawai; Kensuke Arakawa; Yoshiko Honme; Takashi Sasaki; Tadao Saito
Journal:  Appl Environ Microbiol       Date:  2009-08-07       Impact factor: 4.792

9.  Chloromethane-dependent expression of the cmu gene cluster of Hyphomicrobium chloromethanicum.

Authors:  Elena Borodina; Ian R McDonald; J Colin Murrell
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

10.  Conjugative transfer of the integrative conjugative elements ICESt1 and ICESt3 from Streptococcus thermophilus.

Authors:  Xavier Bellanger; Adam P Roberts; Catherine Morel; Frédéric Choulet; Guillaume Pavlovic; Peter Mullany; Bernard Decaris; Gérard Guédon
Journal:  J Bacteriol       Date:  2009-01-30       Impact factor: 3.490
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