Literature DB >> 11688801

Effect of accessory proteins P19 and P20 on cytolytic activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli.

R Manasherob1, A Zaritsky, E Ben-Dov, D Saxena, Z Barak, M Einav.   

Abstract

The gene coding for the accessory protein P19 of Bacillus thuringiensis subsp. israelensis was expressed in Escherichia coli and its product was characterized. To investigate its putative role in delta-endotoxin crystallization as a P20-like polypeptide, each of the two encoding genes, p20 and p19, was cloned for inducible expression coordinatively with cyt1Aa. The latter is known to kill its transgenic host. P20 but not P19 stabilized Cyt1Aa and protected the host cells from its lethal effect. Neither GroEL nor GroES, expressed in trans, affected Cyt1Aa as did P20. The function of P20 is thus more specific than that of the chaperones, but that of P19 remains enigmatic. The correct sequence of p19, confirmed in all five isolates of B. thuringiensis subsp. israelensis, does not explain the slow electrophoretic mobility of its 179 amino acids product.

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Year:  2001        PMID： 11688801     DOI： 10.1007/s002840010316

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  14 in total

1.  Activity of free and clay-bound insecticidal proteins from Bacillus thuringiensis subsp. israelensis against the mosquito Culex pipiens.

Authors:  LanNa Lee; Deepak Saxena; G Stotzky
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

2.  Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis.

Authors:  Colin Berry; Susan O'Neil; Eitan Ben-Dov; Andrew F Jones; Lee Murphy; Michael A Quail; Mathew T G Holden; David Harris; Arieh Zaritsky; Julian Parkhill
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

3.  Partial restoration of antibacterial activity of the protein encoded by a cryptic open reading frame (cyt1Ca) from Bacillus thuringiensis subsp. israelensis by site-directed mutagenesis.

Authors:  Mark Itsko; Robert Manasherob; Arieh Zaritsky
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

4.  Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis.

Authors:  Vladislav Sazhenskiy; Arieh Zaritsky; Mark Itsko
Journal:  Appl Environ Microbiol       Date:  2010-03-26       Impact factor: 4.792

5.  A 1.1-kilobase region downstream of the bin operon in Bacillus sphaericus strain 2362 decreases bin yield and crystal size in strain 2297.

Authors:  Hyun-Woo Park; Mujin Tang; Yuko Sakano; Brian A Federici
Journal:  Appl Environ Microbiol       Date:  2008-12-05       Impact factor: 4.792

6.  Evidence of DNA rearrangements in the 128-kilobase pBtoxis plasmid of Bacillus thuringiensis israelensis.

Authors:  Raida Zribi Zghal; Samir Jaoua
Journal:  Mol Biotechnol       Date:  2006-07       Impact factor: 2.860

7.  Draft genome sequences of two Bacillus thuringiensis strains and characterization of a putative 41.9-kDa insecticidal toxin.

Authors:  Leopoldo Palma; Delia Muñoz; Colin Berry; Jesús Murillo; Primitivo Caballero
Journal:  Toxins (Basel)       Date:  2014-04-30       Impact factor: 4.546

Review 8.  Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.

Authors:  Eitan Ben-Dov
Journal:  Toxins (Basel)       Date:  2014-03-28       Impact factor: 4.546

9.  New Cyt-like δ-endotoxins from Dickeya dadantii: structure and aphicidal activity.

Authors:  Karine Loth; Denis Costechareyre; Géraldine Effantin; Yvan Rahbé; Guy Condemine; Céline Landon; Pedro da Silva
Journal:  Sci Rep       Date:  2015-03-05       Impact factor: 4.379

10.  Draft Genome Sequence of Bacillus thuringiensis Serovar Tolworthi Strain Na205-3, an Isolate Toxic for Helicoverpa armigera.

Authors:  Leopoldo Palma; Delia Muñoz; Jesús Murillo; Primitivo Caballero
Journal:  Genome Announc       Date:  2014-03-13
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