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Literature DB >> 20348307

Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis.

Vladislav Sazhenskiy¹, Arieh Zaritsky, Mark Itsko.

Abstract

The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2010 PMID： 20348307 PMCID： PMC2869120 DOI： 10.1128/AEM.03068-09

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

18 in total

1. A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo.

Authors: H Bujard; R Gentz; M Lanzer; D Stueber; M Mueller; I Ibrahimi; M T Haeuptle; B Dobberstein
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

2. Delta endotoxin of Bacillus thuringiensis subsp. israelensis.

Authors: J L Armstrong; G F Rohrmann; G S Beaudreau
Journal: J Bacteriol Date: 1985-01 Impact factor: 3.490

3. CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus.

Authors: M C Wirth; G P Georghiou; B A Federici
Journal: Proc Natl Acad Sci U S A Date: 1997-09-30 Impact factor: 11.205

4. A detergent-like mechanism of action of the cytolytic toxin Cyt1A from Bacillus thuringiensis var. israelensis.

Authors: Slobodanka D Manceva; Marianne Pusztai-Carey; Paul S Russo; Peter Butko
Journal: Biochemistry Date: 2005-01-18 Impact factor: 3.162

5. Antibacterial activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis.

Authors: Rivka Cahan; Hen Friman; Yeshayahu Nitzan
Journal: Microbiology Date: 2008-11 Impact factor: 2.777

6. Specific targeting to murine myeloma cells of Cyt1Aa toxin from Bacillus thuringiensis subspecies israelensis.

Authors: Shmuel Cohen; Rivka Cahan; Eitan Ben-Dov; Marina Nisnevitch; Arieh Zaritsky; Michael A Firer
Journal: J Biol Chem Date: 2007-07-11 Impact factor: 5.157

7. Sensitivity to plating of Escherichia coli cells expressing the cryA gene from Bacillus thuringiensis var. israelensis.

Authors: J Douek; M Einav; A Zaritsky
Journal: Mol Gen Genet Date: 1992-03

8. A 20-kilodalton protein is required for efficient production of the Bacillus thuringiensis subsp. israelensis 27-kilodalton crystal protein in Escherichia coli.

Authors: L F Adams; J E Visick; H R Whiteley
Journal: J Bacteriol Date: 1989-01 Impact factor: 3.490

9. Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis.

Authors: C Waalwijk; A M Dullemans; M E van Workum; B Visser
Journal: Nucleic Acids Res Date: 1985-11-25 Impact factor: 16.971

10. Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures.

Authors: U Deuschle; W Kammerer; R Gentz; H Bujard
Journal: EMBO J Date: 1986-11 Impact factor: 11.598

4 in total

1. [Larvicidal activity of recombinant Escherichia coli expressing scorpion neurotoxin AaIT or B.t.i toxin Cyt2Ba against mosquito larvae and formulations for enhancing the effects].

Authors: Sheng-Qun Deng; Ming-Zhi Deng; Jia-Ting Chen; Li-Lan Zheng; Hong-Juan Peng
Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2017-06-20