Literature DB >> 1168187

Purification of a fibroblast growth factor from bovine pituitary.

D Gospodarowicz.   

Abstract

The purification from bovine pituitary gland of a growth factor responsible for the control of animal cell division in tissue culture is reported. This growth factor is a polypeptide of 13,300 molecular weight and is homogeneous when analyzed by polyacrylamide gel electrophoresis, carboxymethyl-Sephadex gradient elution chromatography, and Sephadex G-50 chromatography. The yield of growth factor is 5 mg per kg of pituitary. It is active in stimulating DNA synthesis in 3T3 cells at concentrations as low as 2 times 10-13 M with saturation at 1 times 10-10 M.

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Year:  1975        PMID： 1168187

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  97 in total

1.  Media for cultivation of animal cells: an overview.

Authors:  A Mizrahi; A Lazar
Journal:  Cytotechnology       Date:  1988-07       Impact factor: 2.058

Review 2.  Fibroblast growth factors: from molecular evolution to roles in development, metabolism and disease.

Authors:  Nobuyuki Itoh; David M Ornitz
Journal:  J Biochem       Date:  2010-10-12       Impact factor: 3.387

3.  Temporal changes in tectal cell surface specificity induced by nerve growth factor.

Authors:  R Merrell; M W Pulliam; L Randono; L F Boyd; R A Bradshaw; L Glaser
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

4.  Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.

Authors:  H N Antoniades; D Stathakos; C D Scher
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

5.  Characterization of release of basic fibroblast growth factor from bovine retinal endothelial cells in monolayer cultures.

Authors:  R A Brooks; J M Burrin; E M Kohner
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

6.  Interleukin 2 mediates the inhibition of oligodendrocyte progenitor cell proliferation in vitro.

Authors:  R P Saneto; A Altman; R L Knobler; H M Johnson; J de Vellis
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

7.  Pure brain-derived acidic fibroblast growth factor is a potent angiogenic vascular endothelial cell mitogen with sequence homology to interleukin 1.

Authors:  K A Thomas; M Rios-Candelore; G Giménez-Gallego; J DiSalvo; C Bennett; J Rodkey; S Fitzpatrick
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

8.  An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Authors:  H R Bourne; E Rozengurt
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

9.  Transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system.

Authors:  P L Kaplan; M Anderson; B Ozanne
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

10.  In vitro inhibition of the actions of basic FGF by a novel 16 amino acid peptide.

Authors:  I Cosic; A E Drummond; J R Underwood; M T Hearn
Journal:  Mol Cell Biochem       Date:  1994-01-12       Impact factor: 3.396
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