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Literature DB >> 1058478

Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.

Abstract

A basic polypeptide that stimulates DNA synthesis and cell division in confluent populations of mouse Balb/c-3T3 cells has been isolated from whole human serum, and has been separated from the heterogenous group of molecules with insulin-like activity. This highly purified basic polypeptide has a molecular weight of 1.3 x 10(4) and an isoelectric point of 9.7. Approximately 10(7) polypeptide molecules in the growth medium allow the replication of one density-inhibited cell.

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Year: 1975 PMID： 1058478 PMCID： PMC432824 DOI： 10.1073/pnas.72.7.2635

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

1. Studies on the state of insulin in blood: materials and methods for the estimation of "free" and "bound" insulin-like activity in serum.

Authors: H N ANTONIADES; K GUNDERSEN
Journal: Endocrinology Date: 1962-01 Impact factor: 4.736

2. Human somatomedians. Purification and some studies on their biological actions.

Authors: K Uthne
Journal: Acta Endocrinol Suppl (Copenh) Date: 1973

3. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.

Authors: D Gospodarowicz
Journal: Nature Date: 1974-05-10 Impact factor: 49.962

Review 4. The somatomedins: a family of insulinlike hormones under growth hormone control.

Authors: J J Van Wyk; L E Underwood; R L Hintz; D R Clemmons; S J Voina; R P Weaver
Journal: Recent Prog Horm Res Date: 1974

5. The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity.

Authors: R W Pierson; H M Temin
Journal: J Cell Physiol Date: 1972-06 Impact factor: 6.384

6. Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin.

Authors: H M Temin
Journal: J Cell Physiol Date: 1967-06 Impact factor: 6.384

7. Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin.

Authors: D Gospodarowicz; J S Moran
Journal: Proc Natl Acad Sci U S A Date: 1974-11 Impact factor: 11.205

8. Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.

Authors: R W Holley; J A Kiernan
Journal: Proc Natl Acad Sci U S A Date: 1974-08 Impact factor: 11.205

9. Dissociation of cell division stimulating capacity for Balb-c-3T3 from the insulin-like activity in human serum.

Authors: C D Scher; D Stathakos; H N Antoniades
Journal: Nature Date: 1974-02-01 Impact factor: 49.962

10. "Pleiotypic response".

Authors: A Hershko; P Mamont; R Shields; G M Tomkins
Journal: Nat New Biol Date: 1971-08

32 in total

1. An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Authors: H R Bourne; E Rozengurt
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205

Review 2. Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells.

Authors: D Gospodarowicz; G Greenburg; H Bialecki; B R Zetter
Journal: In Vitro Date: 1978-01

Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.

1. Studies on the state of insulin in blood: materials and methods for the estimation of "free" and "bound" insulin-like activity in serum.

2. Human somatomedians. Purification and some studies on their biological actions.

3. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.

Review 4. The somatomedins: a family of insulinlike hormones under growth hormone control.

5. The partial purification from calf serum of a fraction with multiplication-stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity.

6. Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin.

7. Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin.

8. Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.

9. Dissociation of cell division stimulating capacity for Balb-c-3T3 from the insulin-like activity in human serum.

10. "Pleiotypic response".

1. An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Review 2. Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells.

3. A new substrate for cultures of dissociated primary rat brain.

4. An ordered sequence of events is required before BALB/c-3T3 cells become committed to DNA synthesis.

5. Effects of polyprenoic acid on thermal injury.

6. Dialyzable factor in human serum of platelet origin stimulates endothelial cell replication and growth.

7. Proliferative response and oncogene expression induced by epidermal growth factor in EL2 rat fibroblasts.

8. Differential purification of autocrine motility factor derived from a murine protein-free fibrosarcoma.

9. Platelet-derived growth factor promotes proliferation of erythropoietic progenitor cells in vitro.

10. Effects of bovine platelet-poor plasma on the proliferation of normal and transformed BALB/c 3T3 cells.