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Literature DB >> 1159075

Enzymatic degradation of uric acid by uricase-loaded human erythrocytes.

Abstract

Erythrocytes containing pig liver uricase have been prepared by hypotonic hemolysis in the presence of the enzyme. Uricase is shown to be active within the erythrocytes and to degrade uric acid as rapidly as it enters the cells when high intracellular enzyme concentrations are employed. The kinetics and characteristics of uric acid entry are shown to be the same for hemolysed and normal erythrocytes. At physiological concentrations of uric acid, loaded erythrocytes can degrade a maximum of about 21 mumol uric acid/liter erythrocytes per min. The possible application of enzyme-loaded erythrocytes to medicine is discussed.

Entities: Chemical Disease Gene Species

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Year: 1975 PMID： 1159075 PMCID： PMC301907 DOI： 10.1172/JCI108129

Source DB: PubMed Journal: J Clin Invest ISSN： 0021-9738 Impact factor: 14.808

8 in total

1. Studies of the metabolism of human erythrocyte membranes.

Authors: S L SCHRIER
Journal: J Clin Invest Date: 1963-06 Impact factor: 14.808

2. Studies on uricase. I. Preparation, purification, and properties of a cuproprotein.

Authors: H R MAHLER; G HUBSCHER; R BAUM
Journal: J Biol Chem Date: 1955-10 Impact factor: 5.157

3. Studies on uricase. II. The enzyme-substrate complex.

Authors: H BAUM; G HUBSCHER; H R MAHLER
Journal: Biochim Biophys Acta Date: 1956-12

4. Kinetics of uric acid transport in human erythrocytes.

Authors: U V LASSEN
Journal: Biochim Biophys Acta Date: 1961-11-11

5. Hypoxanthine as inhibitor of uric acid transport in human erythrocytes.

Authors: U V LASSEN; K OVERGAARD-HANSEN
Journal: Biochim Biophys Acta Date: 1962-02-12

6. Enzyme loading of erythrocytes.

Authors: G M Ihler; R H Glew; F W Schnure
Journal: Proc Natl Acad Sci U S A Date: 1973-09 Impact factor: 11.205

Review 7. Destruction of injured red cells in vivo.

Authors: R A Rifkind
Journal: Am J Med Date: 1966-11 Impact factor: 4.965

8. The active transport of sodium by ghosts of human red blood cells.

Authors: J F HOFFMAN
Journal: J Gen Physiol Date: 1962-05 Impact factor: 4.086

8 in total

10 in total

Review 1. Drug delivery by red blood cells: vascular carriers designed by mother nature.

Authors: Vladimir R Muzykantov
Journal: Expert Opin Drug Deliv Date: 2010-04 Impact factor: 6.648

Review 2. Erythrocyte-mediated delivery of recombinant enzymes.

Authors: Vincenzo Leuzzi; Luigia Rossi; Claudia Gabucci; Francesca Nardecchia; Mauro Magnani
Journal: J Inherit Metab Dis Date: 2016-03-30 Impact factor: 4.982

3. Biocompatible coupling of therapeutic fusion proteins to human erythrocytes.

Authors: Carlos H Villa; Daniel C Pan; Ian H Johnston; Colin F Greineder; Landis R Walsh; Elizabeth D Hood; Douglas B Cines; Mortimer Poncz; Don L Siegel; Vladimir R Muzykantov
Journal: Blood Adv Date: 2018-02-13

4. Conversion of encapsulated 5-fluoro-2'-deoxyuridine 5'-monophosphate to the antineoplastic drug 5-fluoro-2'-deoxyuridine in human erythrocytes.

Authors: A De Flora; E Zocchi; L Guida; C Polvani; U Benatti
Journal: Proc Natl Acad Sci U S A Date: 1988-05 Impact factor: 11.205

Enzymatic degradation of uric acid by uricase-loaded human erythrocytes.

1. Studies of the metabolism of human erythrocyte membranes.

2. Studies on uricase. I. Preparation, purification, and properties of a cuproprotein.

3. Studies on uricase. II. The enzyme-substrate complex.

4. Kinetics of uric acid transport in human erythrocytes.

5. Hypoxanthine as inhibitor of uric acid transport in human erythrocytes.

6. Enzyme loading of erythrocytes.

Review 7. Destruction of injured red cells in vivo.

8. The active transport of sodium by ghosts of human red blood cells.

Review 1. Drug delivery by red blood cells: vascular carriers designed by mother nature.

Review 2. Erythrocyte-mediated delivery of recombinant enzymes.

3. Biocompatible coupling of therapeutic fusion proteins to human erythrocytes.

4. Conversion of encapsulated 5-fluoro-2'-deoxyuridine 5'-monophosphate to the antineoplastic drug 5-fluoro-2'-deoxyuridine in human erythrocytes.

5. Converting Red Blood Cells to Efficient Microreactors for Blood Detoxification.

6. G-6PD loading of G-6PD-deficient erythrocytes.

7. Organic-acid transport in resealed haemoglobin-containing human erythrocyte 'ghosts'.

8. Immunological evaluation of repeated administration of erythrocyte-entrapped protein to C3H/HeJ mice.

9. Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study.

Review 10. Erythrocytes as Carriers of Therapeutic Enzymes.