| Literature DB >> 11585541 |
D J Johnson1, E N Ostlund, D D Pedersen, B J Schmitt.
Abstract
A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.Entities:
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Year: 2001 PMID: 11585541 PMCID: PMC2631755 DOI: 10.3201/eid0704.010425
Source DB: PubMed Journal: Emerg Infect Dis ISSN: 1080-6040 Impact factor: 6.883