Literature DB >> 11585541

Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

D J Johnson1, E N Ostlund, D D Pedersen, B J Schmitt.   

Abstract

A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.

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Year:  2001        PMID: 11585541      PMCID: PMC2631755          DOI: 10.3201/eid0704.010425

Source DB:  PubMed          Journal:  Emerg Infect Dis        ISSN: 1080-6040            Impact factor:   6.883


  4 in total

1.  Evaluation of immunoglobulin M (IgM) and IgG enzyme immunoassays in serologic diagnosis of West Nile Virus infection.

Authors:  G Tardei; S Ruta; V Chitu; C Rossi; T F Tsai; C Cernescu
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

2.  Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay.

Authors:  R S Lanciotti; A J Kerst; R S Nasci; M S Godsey; C J Mitchell; H M Savage; N Komar; N A Panella; B C Allen; K E Volpe; B S Davis; J T Roehrig
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

3.  Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut.

Authors:  J F Anderson; T G Andreadis; C R Vossbrinck; S Tirrell; E M Wakem; R A French; A E Garmendia; H J Van Kruiningen
Journal:  Science       Date:  1999-12-17       Impact factor: 47.728

4.  Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States.

Authors:  R S Lanciotti; J T Roehrig; V Deubel; J Smith; M Parker; K Steele; B Crise; K E Volpe; M B Crabtree; J H Scherret; R A Hall; J S MacKenzie; C B Cropp; B Panigrahy; E Ostlund; B Schmitt; M Malkinson; C Banet; J Weissman; N Komar; H M Savage; W Stone; T McNamara; D J Gubler
Journal:  Science       Date:  1999-12-17       Impact factor: 47.728

  4 in total
  15 in total

1.  Applications of competitor RNA in diagnostic reverse transcription-PCR.

Authors:  Steven B Kleiboeker
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

2.  Retrospective study of etiologic agents associated with nonsuppurative meningoencephalitis in stranded cetaceans in the canary islands.

Authors:  Eva Sierra; Susan Sánchez; Jeremiah T Saliki; Uriel Blas-Machado; Manuel Arbelo; Daniele Zucca; Antonio Fernández
Journal:  J Clin Microbiol       Date:  2014-04-23       Impact factor: 5.948

3.  Visualization of West Nile Virus in Urine Sediment using Electron Microscopy and Immunogold up to Nine Years Postinfection.

Authors:  Kristy O Murray; Steven Kolodziej; Shannon E Ronca; Rodion Gorchakov; Patricia Navarro; Melissa S Nolan; Amber Podoll; Kevin Finkel; Sreedhar Mandayam
Journal:  Am J Trop Med Hyg       Date:  2017-10-19       Impact factor: 2.345

4.  New sensitive competitive enzyme-linked immunosorbent assay using a monoclonal antibody against nonstructural protein 1 of West Nile virus NY99.

Authors:  Jiro Hirota; Yoshihiro Shimoji; Shinya Shimizu
Journal:  Clin Vaccine Immunol       Date:  2011-12-21

5.  Persistent infection with West Nile virus years after initial infection.

Authors:  Kristy Murray; Christopher Walker; Emily Herrington; Jessica A Lewis; Joseph McCormick; David W C Beasley; Robert B Tesh; Susan Fisher-Hoch
Journal:  J Infect Dis       Date:  2010-01-01       Impact factor: 5.226

Review 6.  West Nile virus: a reemerging global pathogen.

Authors:  L R Petersen; J T Roehrig
Journal:  Emerg Infect Dis       Date:  2001 Jul-Aug       Impact factor: 6.883

7.  West Nile virus encephalitis: sequential histopathological and immunological events in a murine model of infection.

Authors:  David Garcia-Tapia; Daniel E Hassett; William J Mitchell; Gayle C Johnson; Steven B Kleiboeker
Journal:  J Neurovirol       Date:  2007-04       Impact factor: 2.643

8.  West Nile virus isolation from equines in Argentina, 2006.

Authors:  María Alejandra Morales; María Barrandeguy; Cintia Fabbri; Jorge B Garcia; Aldana Vissani; Karina Trono; Gerónimo Gutierrez; Santiago Pigretti; Hernán Menchaca; Nelson Garrido; Nora Taylor; Fernando Fernandez; Silvana Levis; Delia Enría
Journal:  Emerg Infect Dis       Date:  2006-10       Impact factor: 6.883

9.  Equine West Nile encephalitis, United States.

Authors:  E N Ostlund; R L Crom; D D Pedersen; D J Johnson; W O Williams; B J Schmitt
Journal:  Emerg Infect Dis       Date:  2001 Jul-Aug       Impact factor: 6.883

10.  West Nile virus in farmed alligators.

Authors:  Debra L Miller; Michael J Mauel; Charles Baldwin; Gary Burtle; Dallas Ingram; Murray E Hines; Kendal S Frazier
Journal:  Emerg Infect Dis       Date:  2003-07       Impact factor: 6.883

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