Ultrasonic vocalizations as an index of social memory in female mice.

Ultrasonic vocalization (UV) as a measure of social memory was investigated in female mice. UVs emitted by a resident female in the presence of a same-sex partner were measured during a 3-min, pretest social interaction. In a second 3-min test session, mice were reexposed to the familiar partner or presented with a novel partner. In the first case, there was a decline in UVs emitted by resident mice when the intervals between the 2 sessions were 15, 30, or 60 min. After 24 hr, this effect disappeared. In contrast, with a novel female partner, the number of UVs remained unchanged. Scopolamine (0.05 mg/kg ip) disrupted this memory process: Drug-treated females did not show the expected decrease in UVs when reexposed to the familiar female after 30 min. This study provides behavioral and pharmacological evidence that ultrasonic calls can be used as a measure of social memory in female mice.