Literature DB >> 1149742

Improved techniques for the fractionation of non-histone proteins of chromatin on hydroxyapatite.

D Rickwood, A J MacGillivray.   

Abstract

The effect of the dissociation medium on the fractionation of chromatin on hydroxyapatite has been studied. Optimal separations of the histones and non-histone protein are only achieved when columns are run in buffers containing high concentrations of sodium ions. We have modified our previously published method such that the chromosomal proteins can be recovered in virtually quantitative yields. Each of the hydroxyapatite fractions has been analysed with respect to nucleic acid content and the proteins have been analysed by two-dimensional gel electrophoresis.

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Year:  1975        PMID： 1149742     DOI： 10.1111/j.1432-1033.1975.tb03961.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  9 in total

1.  A study of the interaction of histones with DNA using isopycnic centrifugation in metrizamide gradients.

Authors:  D Rickwood; G D Birnie; A J MacGillivray
Journal:  Nucleic Acids Res       Date:  1975-05       Impact factor: 16.971

Review 2.  Mass spectrometry-based strategies for characterization of histones and their post-translational modifications.

Authors:  Xiaodan Su; Chen Ren; Michael A Freitas
Journal:  Expert Rev Proteomics       Date:  2007-04       Impact factor: 3.940

3.  Structural polypeptides of adenovirus type 16 incomplete particles.

Authors:  G Winberg; G Wadell
Journal:  J Virol       Date:  1977-05       Impact factor: 5.103

4.  Changes in liver nuclear sap proteins during chick embryo development.

Authors:  Jan Palyga; Henryk Luboń; Danuta Tyrawska-Spychalowa
Journal:  Wilehm Roux Arch Dev Biol       Date:  1984-01

5.  A note on the use of protease inhibitors during chromatin fractionation on hydroxyapatite columns.

Authors:  M Gaczyński
Journal:  Experientia       Date:  1986-04-15

6.  Major changes in phosphorylation of chromatin-associated non-histone proteins accompany development in the slime mould Dictyostelium discoideum.

Authors:  J H Sinclair; D Rickwood
Journal:  Biochem J       Date:  1985-08-01       Impact factor: 3.857

7.  The isolation and partial characterization of low-molecular-weight phosphorylated component of the non-histone proteins of mouse nuclei.

Authors:  A J MacGillivray; C Johnston; R MacFarlane; D Rickwood
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857

8.  Enrichment and characterization of histones by two-dimensional hydroxyapatite/reversed-phase liquid chromatography-mass spectrometry.

Authors:  Xiaodan Su; Naduparambil K Jacob; Ravindra Amunugama; Pang-Hung Hsu; Richard Fishel; Michael A Freitas
Journal:  Anal Biochem       Date:  2009-02-03       Impact factor: 3.365

9.  Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.

Authors:  Jérôme Govin; Emmanuelle Escoffier; Sophie Rousseaux; Lauriane Kuhn; Myriam Ferro; Julien Thévenon; Raffaella Catena; Irwin Davidson; Jérôme Garin; Saadi Khochbin; Cécile Caron
Journal:  J Cell Biol       Date:  2007-01-29       Impact factor: 10.539
  9 in total

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