Literature DB >> 3007199

A note on the use of protease inhibitors during chromatin fractionation on hydroxyapatite columns.

M Gaczyński.   

Abstract

It was found that NaHSO3 present in the eluents enabled full and reproducible recovery of chromosomal proteins from a hydroxyapatite column. Another protease inhibitor, PMSF, did not have that effect.

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Year:  1986        PMID： 3007199     DOI： 10.1007/bf02118627

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  10 in total

1.  DNA-binding chromosomal non-histone proteins. Isolation, characterization, and tissue specifity.

Authors:  J F Chiu; S Wang; H Fujitani; L S Hnilica
Journal:  Biochemistry       Date:  1975-10-07       Impact factor: 3.162

2.  Preparation and some properties of strongly acidic proteins from calf-thymus nucleohistone.

Authors:  R Hacha; E Fredericq
Journal:  Eur J Biochem       Date:  1975-03-03

3.  Improved techniques for the fractionation of non-histone proteins of chromatin on hydroxyapatite.

Authors:  D Rickwood; A J MacGillivray
Journal:  Eur J Biochem       Date:  1975-02-21

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Chromatin-bound protease: degradation of chromosomal proteins under chromatin dissociation conditions.

Authors:  D B Carter; C B Chae
Journal:  Biochemistry       Date:  1976-01-13       Impact factor: 3.162

6.  A procedure for the isolation of enzymically active rat-liver nuclei.

Authors:  C C Widnell; J R Tata
Journal:  Biochem J       Date:  1964-08       Impact factor: 3.857

7.  The non-histone proteins of chromatin, their isolation and composition in a number of tissues.

Authors:  A J MacGillivray; A Cameron; R J Krauze; D Rickwood; J Paul
Journal:  Biochim Biophys Acta       Date:  1972-08-25

8.  Proteins of chromatin in template restriction. II. Specificity of RNA synthesis.

Authors:  T C Spelsberg; L S Hnilica
Journal:  Biochim Biophys Acta       Date:  1971-01-01

9.  The inhibition of chromatin solubilization by proteolytic inhibitors.

Authors:  R C Krueger
Journal:  Biochem Biophys Res Commun       Date:  1983-01-14       Impact factor: 3.575

10.  Fractionation and characterization of chromosomal proteins by the hydroxyapatite dissociation method.

Authors:  K S Bloom; J N Anderson
Journal:  J Biol Chem       Date:  1978-06-25       Impact factor: 5.157
  10 in total

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