Literature DB >> 11483512

PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.

K Ohishi1, N Inoue, T Kinoshita.   

Abstract

Many eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively.

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Year:  2001        PMID： 11483512      PMCID： PMC149153          DOI： 10.1093/emboj/20.15.4088

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  42 in total

1.  Glycosylphosphatidylinositol biosynthesis validated as a drug target for African sleeping sickness.

Authors:  M A Ferguson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-09-26       Impact factor: 11.205

2.  Mutational analysis of the C-terminal signal peptide of bovine liver 5'-nucleotidase for GPI anchoring: a study on the significance of the hydrophilic spacer region.

Authors:  Y Furukawa; K Tsukamoto; H Ikezawa
Journal:  Biochim Biophys Acta       Date:  1997-09-04

Review 3.  Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis.

Authors:  T Kinoshita; N Inoue
Journal:  Curr Opin Chem Biol       Date:  2000-12       Impact factor: 8.822

4.  Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol-anchor attachment: photocrosslinking studies in a cell-free system.

Authors:  J Vidugiriene; S Vainauskas; A E Johnson; A K Menon
Journal:  Eur J Biochem       Date:  2001-04

5.  Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins.

Authors:  K Ohishi; N Inoue; Y Maeda; J Takeda; H Riezman; T Kinoshita
Journal:  Mol Biol Cell       Date:  2000-05       Impact factor: 4.138

6.  PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER.

Authors:  Y Maeda; R Watanabe; C L Harris; Y Hong; K Ohishi; K Kinoshita; T Kinoshita
Journal:  EMBO J       Date:  2001-01-15       Impact factor: 11.598

7.  Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins.

Authors:  D K Sharma; J D Hilley; J D Bangs; G H Coombs; J C Mottram; A K Menon
Journal:  Biochem J       Date:  2000-11-01       Impact factor: 3.857

8.  Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins.

Authors:  U Meyer; M Benghezal; I Imhof; A Conzelmann
Journal:  Biochemistry       Date:  2000-03-28       Impact factor: 3.162

9.  Post-translational GPI lipid anchor modification of proteins in kingdoms of life: analysis of protein sequence data from complete genomes.

Authors:  B Eisenhaber; P Bork; F Eisenhaber
Journal:  Protein Eng       Date:  2001-01

10.  Early events in glycosylphosphatidylinositol anchor addition. substrate proteins associate with the transamidase subunit gpi8p.

Authors:  T D Spurway; J A Dalley; S High; N J Bulleid
Journal:  J Biol Chem       Date:  2001-02-23       Impact factor: 5.157
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  55 in total

1.  Homozygous Phosphatidylinositol Glycan Class T Mutation in an Indian Girl With Multiple Congenital Anomalies-Hypotonia-Seizures Syndrome 3.

Authors:  Dudipala Sai Chandar; Battu Krishna Chaithanya; Mandapuram Prashanthi
Journal:  Cureus       Date:  2021-04-28

Review 2.  Recent progress in synthetic and biological studies of GPI anchors and GPI-anchored proteins.

Authors:  Shichong Yu; Zhongwu Guo; Charlie Johnson; Guofeng Gu; Qiuye Wu
Journal:  Curr Opin Chem Biol       Date:  2013-10-12       Impact factor: 8.822

3.  Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors:  Takahiro Nakane; Kiyohiko Angata; Takashi Sato; Hiroyuki Kaji; Hisashi Narimatsu
Journal:  J Biol Chem       Date:  2019-03-21       Impact factor: 5.157

4.  Mutations in PIGS, Encoding a GPI Transamidase, Cause a Neurological Syndrome Ranging from Fetal Akinesia to Epileptic Encephalopathy.

Authors:  Thi Tuyet Mai Nguyen; Yoshiko Murakami; Kristen M Wigby; Nissan V Baratang; Justine Rousseau; Anik St-Denis; Jill A Rosenfeld; Stephanie C Laniewski; Julie Jones; Alejandro D Iglesias; Marilyn C Jones; Diane Masser-Frye; Angela E Scheuerle; Denise L Perry; Ryan J Taft; Françoise Le Deist; Miles Thompson; Taroh Kinoshita; Philippe M Campeau
Journal:  Am J Hum Genet       Date:  2018-09-27       Impact factor: 11.025

Review 5.  Paroxysmal nocturnal hemoglobinuria.

Authors:  Robert A Brodsky
Journal:  Blood       Date:  2014-09-18       Impact factor: 22.113

6.  Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum of Saccharomyces cerevisiae.

Authors:  Meng Mei; Chao Zhai; Xinzhi Li; Yu Zhou; Wenfang Peng; Lixin Ma; Qinhong Wang; Brent L Iverson; Guimin Zhang; Li Yi
Journal:  J Biol Chem       Date:  2017-10-16       Impact factor: 5.157

Review 7.  Paroxysmal nocturnal haemoglobinuria.

Authors:  Anita Hill; Amy E DeZern; Taroh Kinoshita; Robert A Brodsky
Journal:  Nat Rev Dis Primers       Date:  2017-05-18       Impact factor: 52.329

8.  Glycosylphosphatidylinositol lipid anchoring of plant proteins. Sensitive prediction from sequence- and genome-wide studies for Arabidopsis and rice.

Authors:  Birgit Eisenhaber; Michael Wildpaner; Carolyn J Schultz; Georg H H Borner; Paul Dupree; Frank Eisenhaber
Journal:  Plant Physiol       Date:  2003-12       Impact factor: 8.340

9.  Deficiencies in the endoplasmic reticulum (ER)-membrane protein Gab1p perturb transfer of glycosylphosphatidylinositol to proteins and cause perinuclear ER-associated actin bar formation.

Authors:  Stephen J Grimme; Xiang-Dong Gao; Paul S Martin; Kim Tu; Serguei E Tcheperegine; Kathleen Corrado; Anne E Farewell; Peter Orlean; Erfei Bi
Journal:  Mol Biol Cell       Date:  2004-04-09       Impact factor: 4.138

10.  Different sets of QTLs influence fitness variation in yeast.

Authors:  Gal Hagit Romano; Yonat Gurvich; Ofer Lavi; Igor Ulitsky; Ron Shamir; Martin Kupiec
Journal:  Mol Syst Biol       Date:  2010-02-16       Impact factor: 11.429
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