Literature DB >> 11430752

Measurement of homonuclear three-bond J(H(N)Halpha) coupling constants in unlabeled peptides complexed with labeled proteins: application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV).

D O Cicero1, G Barbato, U Koch, P Ingallinella, E Bianchi, S Sambucini, P Neddermann, R De Francesco, A Pessi, R Bazzo.   

Abstract

A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(H(N)Halpha) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the 'quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV).

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Year:  2001        PMID： 11430752     DOI： 10.1023/a:1011281527761

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  16 in total

1.  Intensity modulated HSQC and HMQC: two simple methods to measure 3J(HNH)alpha in proteins.

Authors:  P Permi; I Kilpeläinen; A Annila; S Heikkinen
Journal:  J Biomol NMR       Date:  2000-01       Impact factor: 2.835

Review 2.  Pulsed-field gradients: theory and practice.

Authors:  J Keeler; R T Clowes; A L Davis; E D Laue
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

3.  (H)NCAHA and (H)CANNH experiments for the determination of the vicinal coupling constants related to the phi-torsion angle.

Authors:  F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1995-01       Impact factor: 2.835

4.  The solution structure of the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism.

Authors:  G Barbato; D O Cicero; M C Nardi; C Steinkühler; R Cortese; R De Francesco; R Bazzo
Journal:  J Mol Biol       Date:  1999-06-04       Impact factor: 5.469

5.  Structural characterization of the interactions of optimized product inhibitors with the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein by NMR and modelling studies.

Authors:  D O Cicero; G Barbato; U Koch; P Ingallinella; E Bianchi; M C Nardi; C Steinkühler; R Cortese; V Matassa; R De Francesco; A Pessi; R Bazzo
Journal:  J Mol Biol       Date:  1999-06-04       Impact factor: 5.469

6.  On the size of the active site in proteases. I. Papain.

Authors:  I Schechter; A Berger
Journal:  Biochem Biophys Res Commun       Date:  1967-04-20       Impact factor: 3.575

7.  Studies on the solution conformation of human thioredoxin using heteronuclear 15N-1H nuclear magnetic resonance spectroscopy.

Authors:  J E Forman-Kay; A M Gronenborn; L E Kay; P T Wingfield; G M Clore
Journal:  Biochemistry       Date:  1990-02-13       Impact factor: 3.162

8.  Precise vicinal coupling constants 3JHN alpha in proteins from nonlinear fits of J-modulated [15N,1H]-COSY experiments.

Authors:  M Billeter; D Neri; G Otting; Y Q Qian; K Wüthrich
Journal:  J Biomol NMR       Date:  1992-05       Impact factor: 2.835

9.  Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.

Authors:  N A Farrow; R Muhandiram; A U Singer; S M Pascal; C M Kay; G Gish; S E Shoelson; T Pawson; J D Forman-Kay; L E Kay
Journal:  Biochemistry       Date:  1994-05-17       Impact factor: 3.162

10.  Rapid measurement of scalar three-bond 1HN-1H alpha spin coupling constants in 15N-labelled proteins.

Authors:  H Ponstingl; G Otting
Journal:  J Biomol NMR       Date:  1998-08       Impact factor: 2.835
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