Literature DB >> 11398416

Acylated homoserine lactone detection in Pseudomonas aeruginosa biofilms by radiolabel assay.

A L Schaefer1, E P Greenberg, M R Parsek.   

Abstract

We describe the development of a new radioactive assay for acyl-HSL production by bacterial cultures. The assay is based on the uptake of radiolabeled methionine and conversion of the radiolabel into SAM. The radiolabeled SAM is then incorporated into acyl-HSL by an acyl-HSL synthase. This assay is faster than previously used bioassays and shows no bias for the detection of acyl-HSLs of a particular length or side chain substitution. Acyl-HSL production can be monitored over a wide range of growth conditions in liquid culture. This assay can also be used in conjunction with a tube biofilm reactor to monitor acyl-HSL production by biofilm cultures. Ultimately this assay will allow comparison of acyl-HSL production by cells subjected to a variety of physiological conditions.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11398416     DOI： 10.1016/s0076-6879(01)36576-x

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  26 in total

1.  Early activation of quorum sensing.

Authors:  James P Pearson
Journal:  J Bacteriol       Date:  2002-05       Impact factor: 3.490

2.  Influence of the hydrodynamic environment on quorum sensing in Pseudomonas aeruginosa biofilms.

Authors:  Mary Jo Kirisits; Jeffrey J Margolis; Boloroo L Purevdorj-Gage; Benjamin Vaughan; David L Chopp; Paul Stoodley; Matthew R Parsek
Journal:  J Bacteriol       Date:  2007-08-17       Impact factor: 3.490

3.  Isovaleryl-homoserine lactone, an unusual branched-chain quorum-sensing signal from the soybean symbiont Bradyrhizobium japonicum.

Authors:  Andrea Lindemann; Gabriella Pessi; Amy L Schaefer; Margrith E Mattmann; Quin H Christensen; Aline Kessler; Hauke Hennecke; Helen E Blackwell; E Peter Greenberg; Caroline S Harwood
Journal:  Proc Natl Acad Sci U S A       Date:  2011-09-26       Impact factor: 11.205

4.  Characterization of colony morphology variants isolated from Pseudomonas aeruginosa biofilms.

Authors:  Mary Jo Kirisits; Lynne Prost; Melissa Starkey; Matthew R Parsek
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

5.  "Hot Stuff": The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals.

Authors:  Amy L Schaefer; Caroline S Harwood; E Peter Greenberg
Journal:  Methods Mol Biol       Date:  2018

6.  Multiple N-acyl homoserine lactone signals of Rhizobium leguminosarum are synthesized in a distinct temporal pattern.

Authors:  R S Blosser-Middleton; K M Gray
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

7.  Sialylation of lipooligosaccharides promotes biofilm formation by nontypeable Haemophilus influenzae.

Authors:  W Edward Swords; Miranda L Moore; Luciana Godzicki; Gail Bukofzer; Michael J Mitten; Jessica VonCannon
Journal:  Infect Immun       Date:  2004-01       Impact factor: 3.441

8.  Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation.

Authors:  Kara D Jackson; Melissa Starkey; Stefanie Kremer; Matthew R Parsek; Daniel J Wozniak
Journal:  J Bacteriol       Date:  2004-07       Impact factor: 3.490

9.  The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone.

Authors:  Breck A Duerkop; Jake P Herman; Ricky L Ulrich; Mair E A Churchill; E Peter Greenberg
Journal:  J Bacteriol       Date:  2008-05-16       Impact factor: 3.490

10.  Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix.

Authors:  Bradley R Borlee; Aaron D Goldman; Keiji Murakami; Ram Samudrala; Daniel J Wozniak; Matthew R Parsek
Journal:  Mol Microbiol       Date:  2010-01-17       Impact factor: 3.501
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.