Literature DB >> 29130162

"Hot Stuff": The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals.

Amy L Schaefer1, Caroline S Harwood2, E Peter Greenberg3.   

Abstract

Many Proteobacteria synthesize acyl-homoserine lactone (AHL) molecules for use as signals in cell density-dependent gene regulation known as quorum sensing (QS) and response. AHL detection protocols are essential to QS researchers and several techniques are available, including a 14C-AHL radiolabel assay. This assay is based on the uptake of radiolabeled methionine by living cells and conversion of the radiolabel into S-adenosylmethionine (SAM). The radiolabeled SAM is then incorporated into AHL signal by an AHL synthase enzyme. Here we describe a methodology to perform the AHL radiolabel assay, which is unbiased, relatively fast, and very sensitive compared to other AHL detection protocols.

Entities:  

Keywords:  14C-carboxy-methionine; AHL; Acyl-HSL; Acyl-homoserine lactone; LuxI; QS; Quorum sensing

Mesh：

Substances：

Year:  2018        PMID： 29130162      PMCID： PMC5805386          DOI： 10.1007/978-1-4939-7309-5_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  34 in total

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