Literature DB >> 11381134

Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes.

J McKinney1, C Guerrier-Takada, D Wesolowski, S Altman.   

Abstract

Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (EGS) have been designed to complex with either mRNA. Using the EGS technology, the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA. In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.

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Year:  2001        PMID： 11381134      PMCID： PMC34400          DOI： 10.1073/pnas.121180398

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

Review 1.  Microbial genome sequencing.

Authors:  C M Fraser; J A Eisen; S L Salzberg
Journal:  Nature       Date:  2000-08-17       Impact factor: 49.962

Review 2.  The crisis in antibiotic resistance.

Authors:  H C Neu
Journal:  Science       Date:  1992-08-21       Impact factor: 47.728

Review 3.  Epidemiology of drug resistance: implications for a post-antimicrobial era.

Authors:  M L Cohen
Journal:  Science       Date:  1992-08-21       Impact factor: 47.728

4.  External guide sequences for an RNA enzyme.

Authors:  A C Forster; S Altman
Journal:  Science       Date:  1990-08-17       Impact factor: 47.728

5.  DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.

Authors:  Y Yang; G F Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

6.  Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.

Authors:  S L Swanberg; J C Wang
Journal:  J Mol Biol       Date:  1987-10-20       Impact factor: 5.469

7.  Structure of the Drosophila DNA topoisomerase II gene. Nucleotide sequence and homology among topoisomerases II.

Authors:  E Wyckoff; D Natalie; J M Nolan; M Lee; T Hsieh
Journal:  J Mol Biol       Date:  1989-01-05       Impact factor: 5.469

8.  Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli.

Authors:  H Yoshida; M Bogaki; M Nakamura; S Nakamura
Journal:  Antimicrob Agents Chemother       Date:  1990-06       Impact factor: 5.191

9.  Cloning and characterization of a DNA gyrase A gene from Escherichia coli that confers clinical resistance to 4-quinolones.

Authors:  M E Cullen; A W Wyke; R Kuroda; L M Fisher
Journal:  Antimicrob Agents Chemother       Date:  1989-06       Impact factor: 5.191

10.  Targeted cleavage of mRNA in vitro by RNase P from Escherichia coli.

Authors:  Y Li; C Guerrier-Takada; S Altman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205
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  19 in total

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Authors:  Jeffrey McKinney; Cecilia Guerrier-Takada; Jorge Galán; Sidney Altman
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490

2.  Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA.

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Journal:  RNA       Date:  2010-09-03       Impact factor: 4.942

Review 3.  Inhibition of gene expression in human cells using RNase P-derived ribozymes and external guide sequences.

Authors:  Kihoon Kim; Fenyong Liu
Journal:  Biochim Biophys Acta       Date:  2007-09-29

4.  Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences.

Authors:  Eirik W Lundblad; Gaoping Xiao; Jae-Hyeong Ko; Sidney Altman
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-08       Impact factor: 11.205

5.  Impact of DNA gyrase inhibition by antisense ribozymes on rec A in E. coli.

Authors:  Sainath Rao Shilpakala; Malathi Raghunathan
Journal:  Mol Biol Rep       Date:  2008-11-04       Impact factor: 2.316

6.  Basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes.

Authors:  Donna Wesolowski; Hyun Seop Tae; Neeru Gandotra; Paula Llopis; Ning Shen; Sidney Altman
Journal:  Proc Natl Acad Sci U S A       Date:  2011-09-26       Impact factor: 11.205

7.  Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in Plasmodium falciparum.

Authors:  Aprajita Garg; Donna Wesolowski; Dulce Alonso; Kirk W Deitsch; Choukri Ben Mamoun; Sidney Altman
Journal:  Proc Natl Acad Sci U S A       Date:  2015-09-08       Impact factor: 11.205

Review 8.  External guide sequence technology: a path to development of novel antimicrobial therapeutics.

Authors:  Carol Davies-Sala; Alfonso Soler-Bistué; Robert A Bonomo; Angeles Zorreguieta; Marcelo E Tolmasky
Journal:  Ann N Y Acad Sci       Date:  2015-04-09       Impact factor: 5.691

9.  Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences.

Authors:  Jeffrey S McKinney; Haifeng Zhang; Tomoko Kubori; Jorge E Galán; Sidney Altman
Journal:  Nucleic Acids Res       Date:  2004-02-03       Impact factor: 16.971

10.  Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans.

Authors:  Qitao Yan; Rui Zhao; Wenlin Zheng; Changxin Yin; Bao Zhang; Wenli Ma
Journal:  BMC Biotechnol       Date:  2009-05-20       Impact factor: 2.563
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