Literature DB >> 11376077

Comparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs.

M Chernesky1, D Jang, D Copes, J Patel, A Petrich, K Biers, A Sproston, J Kapala.   

Abstract

Two endocervical swabs from each of 1,123 women were collected into manufacturer-supplied transport tubes and tested for Chlamydia trachomatis by a polymer conjugate-enhanced (PCE) enzyme immunoassay (EIA) (IDEIA PCE Chlamydia; DAKO) and a ligase chain reaction assay (LCx Chlamydia; Abbott). After confirmation by the EIA blocking test, the sensitivity of the IDEIA PCE remained at 91.8% and the specificity increased from 98.2 to 99.8% compared to LCx.

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Year:  2001        PMID： 11376077      PMCID： PMC88131          DOI： 10.1128/JCM.39.6.2306-2307.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Chlamydia trachomatis confirmatory testing of PCR-positive genitourinary specimens using a second set of plasmid primers.

Authors:  J B Mahony; K E Luinstra; D Jang; J Sellors; M A Chernesky
Journal:  Mol Cell Probes       Date:  1992-10       Impact factor: 2.365

2.  Noninvasive tests for diagnosis of Chlamydia trachomatis infection: application of ligase chain reaction to first-catch urine specimens of women.

Authors:  J Schachter; J Moncada; R Whidden; H Shaw; G Bolan; J D Burczak; H H Lee
Journal:  J Infect Dis       Date:  1995-11       Impact factor: 5.226

Review 3.  DFA, EIA, PCR, LCR and other technologies: what tests should be used for diagnosis of chlamydia infections?

Authors:  J Schachter
Journal:  Immunol Invest       Date:  1997 Jan-Feb       Impact factor: 3.657

4.  Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay.

Authors:  M A Chernesky; H Lee; J Schachter; J D Burczak; W E Stamm; W M McCormack; T C Quinn
Journal:  J Infect Dis       Date:  1994-11       Impact factor: 5.226

5.  Diagnosis of genitourinary Chlamydia trachomatis infections by using the ligase chain reaction on patient-obtained vaginal swabs.

Authors:  E W Hook; K Smith; C Mullen; J Stephens; L Rinehardt; M S Pate; H H Lee
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

6.  The vaginal introitus: a novel site for Chlamydia trachomatis testing in women.

Authors:  H C Wiesenfeld; R P Heine; A Rideout; I Macio; F DiBiasi; R L Sweet
Journal:  Am J Obstet Gynecol       Date:  1996-05       Impact factor: 8.661

7.  Vulval swabs as alternative specimens for ligase chain reaction detection of genital chlamydial infection in women.

Authors:  A Stary; B Najim; H H Lee
Journal:  J Clin Microbiol       Date:  1997-04       Impact factor: 5.948

8.  Comparison of the Syva MicroTrak enzyme immunoassay and Gen-Probe PACE 2 with cell culture for diagnosis of cervical Chlamydia trachomatis infection in a high-prevalence female population.

Authors:  L M Clarke; M F Sierra; B J Daidone; N Lopez; J M Covino; W M McCormack
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

9.  Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction.

Authors:  M A Chernesky; D Jang; H Lee; J D Burczak; H Hu; J Sellors; S J Tomazic-Allen; J B Mahony
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

10.  Comparative evaluation of chlamydiazyme, PACE 2, and AMP-CT assays for detection of Chlamydia trachomatis in endocervical specimens.

Authors:  J L Wylie; S Moses; R Babcock; A Jolly; S Giercke; G Hammond
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948
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  5 in total

1.  Chlamydia trachomatis diagnostics.

Authors:  M A Chernesky
Journal:  Sex Transm Infect       Date:  2002-08       Impact factor: 3.519

Review 2.  [Modern diagnosis of Chlamydia trachomatis infections].

Authors:  T Meyer
Journal:  Hautarzt       Date:  2007-01       Impact factor: 0.751

3.  Enhanced enzyme immunoassay with negative-gray-zone testing compared to a single nucleic Acid amplification technique for community-based chlamydial screening of men.

Authors:  Paddy Horner; Sue Skidmore; Alan Herring; Jo Sell; Ian Paul; Owen Caul; Matthias Egger; Anne McCarthy; Emma Sanford; Chris Salisbury; John Macleod; Jonathan Sterne; Nicola Low
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

Review 4.  Diagnosis and assessment of trachoma.

Authors:  Anthony W Solomon; Rosanna W Peeling; Allen Foster; David C W Mabey
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

5.  The superiority of polymerase chain reaction over an amplified enzyme immunoassay for the detection of genital chlamydial infections.

Authors:  H Jalal; H Stephen; A Al-Suwaine; C Sonnex; C Carne
Journal:  Sex Transm Infect       Date:  2006-02       Impact factor: 3.519
  5 in total

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