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Literature DB >> 1125192

Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III.

Abstract

DNA-dependent RNA polymerase III (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.-7.6) has been isolated and partially purified from calf thymus tissue. Significant amounts of enzyme III are present in this tissue (up to 15% of the total activity of thymus homogenates). This enzyme has been characterized with respect to its chromatographic properties, broad ammonium sulfate optimum (0.04-0.2 M), template requirements, divalent metal optima, and its unique alpha-amanitin sensitivity (50% inhibition of activity occurring at an alpha-amanitin concentration of 10 mug/ml).

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Year: 1975 PMID： 1125192 DOI： 10.1021/bi00679a015

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

15 in total

1. The sensitivity of RNA polymerases I and II from Novikoff hepatoma (N1S1) cells to 3'-deoxyadenosine 5'-triphosphate.

Authors: R C Desrosiers; F M Rottman; J A Boezi; H C Towle
Journal: Nucleic Acids Res Date: 1976-02 Impact factor: 16.971

2. [Free and template-bound RNA polymerase in normal and leukaemic human lymphocytes (author's transl)].

Authors: M Garbrecht; R Mertelsmann
Journal: Klin Wochenschr Date: 1976-03-01

3. Purification of two transcription factors required for initiation by mammalian RNA polymerase II.

Authors: T Kawaguchi; S Kitajima; Y Niho; T Oda; J Germino; S M Weissman; Y Yasukochi
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

4. Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

Authors: R Landick; A Colwell; J Stewart
Journal: J Bacteriol Date: 1990-06 Impact factor: 3.490

5. Identification of two transcription factors that bind to specific elements in the promoter of the adenovirus early-region 4.

Authors: H Watanabe; T Imai; P A Sharp; H Handa
Journal: Mol Cell Biol Date: 1988-03 Impact factor: 4.272

Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III.

1. The sensitivity of RNA polymerases I and II from Novikoff hepatoma (N1S1) cells to 3'-deoxyadenosine 5'-triphosphate.

2. [Free and template-bound RNA polymerase in normal and leukaemic human lymphocytes (author's transl)].

3. Purification of two transcription factors required for initiation by mammalian RNA polymerase II.

4. Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

5. Identification of two transcription factors that bind to specific elements in the promoter of the adenovirus early-region 4.

6. Specific termination of in vitro transcription by calf thymus RNA polymerase III.

7. Inhibitory monoclonal antibody to calf thymus RNA polymerase II blocks formation of enzyme-DNA complexes.

8. An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.

9. Effect of estrogen on gene expression in chicken oviduct: evidence for transcriptional control of ovalbumin gene.

10. Purification and Characterization of DNA-dependent RNA Polymerases from Cauliflower Nuclei.