Literature DB >> 2395865

Purification of two transcription factors required for initiation by mammalian RNA polymerase II.

T Kawaguchi1, S Kitajima, Y Niho, T Oda, J Germino, S M Weissman, Y Yasukochi.   

Abstract

We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of approximately 33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.

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Year:  1990        PMID： 2395865      PMCID： PMC54588          DOI： 10.1073/pnas.87.17.6619

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  38 in total

1.  Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III.

Authors:  P A Weil; S P Blatti
Journal:  Biochemistry       Date:  1975-04-22       Impact factor: 3.162

2.  DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.

Authors:  D J Galas; A Schmitz
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.

Authors:  M Horikoshi; C K Wang; H Fujii; J A Cromlish; P A Weil; R G Roeder
Journal:  Nature       Date:  1989-09-28       Impact factor: 49.962

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Five intermediate complexes in transcription initiation by RNA polymerase II.

Authors:  S Buratowski; S Hahn; L Guarente; P A Sharp
Journal:  Cell       Date:  1989-02-24       Impact factor: 41.582

7.  Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.

Authors:  O Flores; E Maldonado; D Reinberg
Journal:  J Biol Chem       Date:  1989-05-25       Impact factor: 5.157

8.  Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.

Authors:  N Nakajima; M Horikoshi; R G Roeder
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

9.  Factors involved in specific transcription by mammalian RNA polymerase II. RNA polymerase II-associating protein 30 is an essential component of transcription factor IIF.

Authors:  O Flores; E Maldonado; Z Burton; J Greenblatt; D Reinberg
Journal:  J Biol Chem       Date:  1988-08-05       Impact factor: 5.157

10.  Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.

Authors:  H G Hodo; S P Blatti
Journal:  Biochemistry       Date:  1977-05-31       Impact factor: 3.162
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  1 in total

1.  Class II (B) general transcription factor (TFIIB) that binds to the template-committed preinitiation complex is different from general transcription factor BTF3.

Authors:  V Moncollin; L Fischer; B Cavallini; J M Egly; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205
  1 in total

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