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Literature DB >> 6283476

An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.

Abstract

A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5' end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotides. Denatured salmon sperm DNA served as the standard template in this reaction. SV40 FIII DNA was also an effective template, SV40 FII DNA was ineffective while FI yielded mainly free decaribonucleotides. The incorporation depended on the other rNTPs and was resistant to high concentrations of alpha-amanitin and rifamycin AF/013, drugs inhibitory to RNA polymerases I, II and III. The results implicate the decaribonucleotide polymerase in the priming of nascent DNA chains and suggest that the unique size of iRNA is encoded within its primase.

Entities: Chemical Disease

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Year: 1982 PMID： 6283476 PMCID： PMC320611 DOI： 10.1093/nar/10.7.2309

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

5. Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.

Authors: K Yoda; T Okazaki
Journal: Nucleic Acids Res Date: 1983-06-11 Impact factor: 16.971

An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.

1. Internal structure of the chromatin subunit.

2. Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III.

3. Priming of superhelical SV40 DNA by Escherichia coli RNA polymerase for in vitro DNA synthesis.

4. Animal DNA-dependent RNA polymerases. 4. Studies on inhibition by rifamycin derivatives.

5. Initiator RNA in discontinuous polyoma DNA synthesis.

6. Alpha-amanitin: a specific inhibitor of one of two DNA-pendent RNA polymerase activities from calf thymus.

7. Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.

8. RNA-linked short DNA fragments during polyoma replication.

9. Role of DNA-dependent RNA polymerase 3 in the transcription of the tRNA and 5S RNA genes.

10. In vitro polyoma DNA synthesis: characterization of a system from infected 3T3 cells.

1. Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.

2. Bidirectional promoter elements of simian virus 40 are required for efficient replication of the viral DNA.

3. Intrinsic coupling of lagging-strand synthesis to chromatin assembly.

4. Mouse primase initiation sites in the origin region of simian virus 40.

5. Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.

6. Polyomavirus minichromosomes: associated DNA topoisomerase II and DNA ligase activities.

7. The mammalian primase is part of a high molecular weight DNA polymerase alpha polypeptide.

8. A mammalian DNA polymerase alpha holoenzyme functioning on defined in vivo-like templates.