Literature DB >> 11243833

A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity.

C Kao1, S Rüdisser, M Zheng.   

Abstract

Ribose C2' methoxy groups (-OCH3) on the penultimate nucleotide or the last two nucleotides of DNA templates can dramatically improve the quality of transcripts produced by T7 RNA polymerase. This strategy can be adapted to generate transcripts of varying lengths and will allow greater ease in purification of the products.

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Year:  2001        PMID： 11243833     DOI： 10.1006/meth.2000.1131

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  26 in total

1.  Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: tailored-SELEX.

Authors:  Axel Vater; Florian Jarosch; Klaus Buchner; Sven Klussmann
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

2.  Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter.

Authors:  Tricia M Coleman; Guocan Wang; Faqing Huang
Journal:  Nucleic Acids Res       Date:  2004-01-15       Impact factor: 16.971

3.  The linear form of a group II intron catalyzes efficient autocatalytic reverse splicing, establishing a potential for mobility.

Authors:  Michael Roitzsch; Anna Marie Pyle
Journal:  RNA       Date:  2009-01-23       Impact factor: 4.942

4.  Solution structure and metal ion binding sites of the human CPEB3 ribozyme's P4 domain.

Authors:  Miriam Skilandat; Magdalena Rowinska-Zyrek; Roland K O Sigel
Journal:  J Biol Inorg Chem       Date:  2014-03-21       Impact factor: 3.358

5.  Linear group II intron RNAs can retrohome in eukaryotes and may use nonhomologous end-joining for cDNA ligation.

Authors:  Fanglei Zhuang; Marta Mastroianni; Travis B White; Alan M Lambowitz
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-15       Impact factor: 11.205

6.  DNA cleavage and reverse splicing of ribonucleoprotein particles reconstituted in vitro with linear RmInt1 RNA.

Authors:  María Dolores Molina-Sánchez; Nicolás Toro
Journal:  RNA Biol       Date:  2019-04-14       Impact factor: 4.652

7.  Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis.

Authors:  Yasushi Hikida; Michiko Kimoto; Shigeyuki Yokoyama; Ichiro Hirao
Journal:  Nat Protoc       Date:  2010-06-24       Impact factor: 13.491

8.  Structural Basis for Substrate Helix Remodeling and Cleavage Loop Activation in the Varkud Satellite Ribozyme.

Authors:  Saurja DasGupta; Nikolai B Suslov; Joseph A Piccirilli
Journal:  J Am Chem Soc       Date:  2017-07-03       Impact factor: 15.419

Review 9.  Advances in methods for native expression and purification of RNA for structural studies.

Authors:  Robert T Batey
Journal:  Curr Opin Struct Biol       Date:  2014-02-28       Impact factor: 6.809

10.  The 2'-OH group at the group II intron terminus acts as a proton shuttle.

Authors:  Michael Roitzsch; Olga Fedorova; Anna Marie Pyle
Journal:  Nat Chem Biol       Date:  2010-01-31       Impact factor: 15.040
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