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Literature DB >> 11214178

A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation.

Abstract

In vitro selection was used to sample SnRNA-related sequences for ribozyme activities, and several 2',5'-branch-forming ribozymes were isolated. One such ribozyme is highly dependent upon an 11-nt motif that contains a conserved U6 snRNA sequence (ACAGAGA-box) known to be important for pre-mRNA splicing. The ribozyme reaction is similar to the first step of splicing in that an internal 2'-hydroxyl of an unpaired adenosine attacks at the 5'-phosphate of a guanosine. It differs in that the leaving group is diphosphate rather than a 5' exon. The finding that lariat formation can be accomplished by a small RNA with sequences related to U6 snRNA indicates that the RNA available in the spliceosome may be involved in RNA-catalyzed branch formation.

Entities: Chemical

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Year: 2001 PMID： 11214178 PMCID： PMC1370066 DOI： 10.1017/s1355838201001510

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

67 in total

1. Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.

Authors: V Ségault; C L Will; M Polycarpou-Schwarz; I W Mattaj; C Branlant; R Lührmann
Journal: Mol Cell Biol Date: 1999-04 Impact factor: 4.272

A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation.

1. Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.

2. More mistakes by T7 RNA polymerase at the 5' ends of in vitro-transcribed RNAs.

3. The generality of self-splicing RNA: relationship to nuclear mRNA splicing.

Review 4. On the origin of RNA splicing and introns.

Review 5. Mechanical devices of the spliceosome: motors, clocks, springs, and things.

6. Accessing rare activities from random RNA sequences: the importance of the length of molecules in the starting pool.

Review 7. Classification of introns: U2-type or U12-type.

8. Genetic interaction between U6 snRNA and the first intron nucleotide in Saccharomyces cerevisiae.

9. Metal ion catalysis during group II intron self-splicing: parallels with the spliceosome.

10. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes.

1. Practical and general synthesis of 5'-adenylated RNA (5'-AppRNA).

2. In vitro selection of an archaeal RNase P RNA mimics natural variation.

3. A critical assessment of the utility of protein-free splicing systems.

4. Identification and characterization of a short 2'-3' bond-forming ribozyme.

Review 5. Model systems: how chemical biologists study RNA.

6. An evaluation of detection methods for large lariat RNAs.

7. Inhibition of pre-mRNA splicing by synthetic branched nucleic acids.

Review 8. Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.

9. Structure-function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase.