Literature DB >> 11164957

Development of a technology for automation and miniaturization of protein crystallization.

U Mueller1, L Nyarsik, M Horn, H Rauth, T Przewieslik, W Saenger, H Lehrach, H Eickhoff.   

Abstract

The usage of standard 96 well microplates for the screening of crystallization conditions of recombinant proteins offers several advantages when compared to commonly used crystallization plate formats. The adoption of robotic technology for plate and glass slide preparation within a "hanging drop" vapour diffusion crystallization experiment enables to work with an increased throughput at reduced costs. In addition to commercial pipetting devices with a 96-channel aspirator/dispenser, solenoid ink-jet technology was applied to form 250 nl droplets with a diameter of approximately 1 mm. This allows miniaturization of crystallization screening set-ups with an estimated ten-fold cost reduction when compared to commonly used 24 well plates.

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Year:  2001        PMID： 11164957     DOI： 10.1016/s0168-1656(00)00349-7

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  9 in total

1.  A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion.

Authors:  Carl L Hansen; Emmanuel Skordalakes; James M Berger; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-16       Impact factor: 11.205

Review 2.  Protein crystallization for genomics: throughput versus output.

Authors:  Naomi E Chayen
Journal:  J Struct Funct Genomics       Date:  2003

3.  Rapid purification and crystal structure analysis of a small protein carrying two terminal affinity tags.

Authors:  Uwe Mueller; Konrad Büssow; Anne Diehl; Franz J Bartl; Frank H Niesen; Lajos Nyarsik; Udo Heinemann
Journal:  J Struct Funct Genomics       Date:  2003

4.  Lessons from high-throughput protein crystallization screening: 10 years of practical experience.

Authors:  Joseph R Luft; Edward H Snell; George T Detitta
Journal:  Expert Opin Drug Discov       Date:  2011-03-22       Impact factor: 6.098

5.  The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex.

Authors:  Daniel Kümmel; Jürgen J Müller; Yvette Roske; Rolf Misselwitz; Konrad Büssow; Udo Heinemann
Journal:  EMBO Rep       Date:  2005-08       Impact factor: 8.807

6.  Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima.

Authors:  Carole Barbey; Nicolas Rouhier; Ahmed Haouz; Alda Navaza; Jean-Pierre Jacquot
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-12-20

7.  Trace fluorescent labeling for high-throughput crystallography.

Authors:  Elizabeth Forsythe; Aniruddha Achari; Marc L Pusey
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-02-22

8.  Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization.

Authors:  Andrew P Turnbull; Daniel Kümmel; Bianka Prinz; Caterina Holz; Jeffrey Schultchen; Christine Lang; Frank H Niesen; Klaus-Peter Hofmann; Heinrich Delbrück; Joachim Behlke; Eva-Christina Müller; Ernst Jarosch; Thomas Sommer; Udo Heinemann
Journal:  EMBO J       Date:  2005-02-03       Impact factor: 11.598

9.  X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1).

Authors:  Babu A Manjasetty; Frank H Niesen; Christoph Scheich; Yvette Roske; Frank Goetz; Joachim Behlke; Volker Sievert; Udo Heinemann; Konrad Büssow
Journal:  BMC Struct Biol       Date:  2005-12-14
  9 in total

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