Literature DB >> 14649295

Protein crystallization for genomics: throughput versus output.

Naomi E Chayen1.   

Abstract

Structural genomics involves many steps in order to reach from Gene to structure. This article focuses on the crystallization step in this chain of tasks. It is becoming increasingly evident that the current high throughput procedures for crystallising proteins do not always produce the expected output of high quality crystals required for structure determination by x-ray crystallography. This problem is discussed and suggestions for raising the output are presented.

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Year:  2003        PMID： 14649295     DOI： 10.1023/a:1026174727482

Source DB:  PubMed          Journal:  J Struct Funct Genomics        ISSN： 1345-711X


  17 in total

Review 1.  Automation of X-ray crystallography.

Authors:  E Abola; P Kuhn; T Earnest; R C Stevens
Journal:  Nat Struct Biol       Date:  2000-11

Review 2.  High-throughput crystallization and structure determination in drug discovery.

Authors:  Lance Stewart; Robin Clark; Craig Behnke
Journal:  Drug Discov Today       Date:  2002-02-01       Impact factor: 7.851

3.  Tackling the bottleneck of protein crystallization in the post-genomic era.

Authors:  Naomi E Chayen
Journal:  Trends Biotechnol       Date:  2002-03       Impact factor: 19.536

4.  Development of a technology for automation and miniaturization of protein crystallization.

Authors:  U Mueller; L Nyarsik; M Horn; H Rauth; T Przewieslik; W Saenger; H Lehrach; H Eickhoff
Journal:  J Biotechnol       Date:  2001-01-23       Impact factor: 3.307

5.  High-throughput screens for postgenomics: studies of protein crystallization using microsystems technology.

Authors:  G Juárez-Martínez; P Steinmann; A W Roszak; N W Isaacs; J M Cooper
Journal:  Anal Chem       Date:  2002-07-15       Impact factor: 6.986

6.  Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques.

Authors:  Juan Ma Garcia-Ruíz; Luis Antonio Gonzalez-Ramirez; José Antonio Gavira; Fermín Otálora
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-09-26

7.  A novel technique for containerless protein crystallization.

Authors:  N E Chayen
Journal:  Protein Eng       Date:  1996-10

Review 8.  Comparative studies of protein crystallization by vapour-diffusion and microbatch techniques.

Authors:  N E Chayen
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-01-01

9.  The role of oil in macromolecular crystallization.

Authors:  N E Chayen
Journal:  Structure       Date:  1997-10-15       Impact factor: 5.006

10.  Parameters for crystal growth of ribosomal subunits.

Authors:  A Yonath; J Müssig; H G Wittmann
Journal:  J Cell Biochem       Date:  1982       Impact factor: 4.429
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  2 in total

1.  Polarization-resolved second-harmonic generation microscopy as a method to visualize protein-crystal domains.

Authors:  Emma L DeWalt; Victoria J Begue; Judith A Ronau; Shane Z Sullivan; Chittaranjan Das; Garth J Simpson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-12-20

2.  Crystal structure of an uncommon cellulosome-related protein module from Ruminococcus flavefaciens that resembles papain-like cysteine peptidases.

Authors:  Maly Levy-Assaraf; Milana Voronov-Goldman; Inna Rozman Grinberg; Gloria Weiserman; Linda J W Shimon; Sadanari Jindou; Ilya Borovok; Bryan A White; Edward A Bayer; Raphael Lamed; Felix Frolow
Journal:  PLoS One       Date:  2013-02-14       Impact factor: 3.240
  2 in total

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