Literature DB >> 11158149

Quantification of human cytomegalovirus DNA by real-time PCR.

E Gault1, Y Michel, A Dehée, C Belabani, J C Nicolas, A Garbarg-Chenon.   

Abstract

A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).

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Year:  2001        PMID： 11158149      PMCID： PMC87818          DOI： 10.1128/JCM.39.2.772-775.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation.

Authors:  U Machida; M Kami; T Fukui; Y Kazuyama; M Kinoshita; Y Tanaka; Y Kanda; S Ogawa; H Honda; S Chiba; K Mitani; Y Muto; K Osumi; S Kimura; H Hirai
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Detection of human cytomegalovirus DNA by real-time quantitative PCR.

Authors:  A Nitsche; N Steuer; C A Schmidt; O Landt; H Ellerbrok; G Pauli; W Siegert
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Real-time monitoring of cytomegalovirus infections after stem cell transplantation using the TaqMan polymerase chain reaction assays.

Authors:  Z Yun; I Lewensohn-Fuchs; P Ljungman; A Vahlne
Journal:  Transplantation       Date:  2000-04-27       Impact factor: 4.939

4.  Effect of delayed specimen processing on cytomegalovirus antigenemia test results.

Authors:  M L Landry; D Ferguson; S Cohen; K Huber; P Wetherill
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

5.  Quantitative analysis of cytomegalovirus load using a real-time PCR assay.

Authors:  N Tanaka; H Kimura; K Iida; Y Saito; I Tsuge; A Yoshimi; T Matsuyama; T Morishima
Journal:  J Med Virol       Date:  2000-04       Impact factor: 2.327

6.  Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA.

Authors:  A Nitsche; N Steuer; C A Schmidt; O Landt; W Siegert
Journal:  Clin Chem       Date:  1999-11       Impact factor: 8.327

7.  Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation.

Authors:  E Drouet; R Colimon; S Michelson; N Fourcade; A Niveleau; C Ducerf; A Boibieux; M Chevallier; G Denoyel
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

8.  Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients.

Authors:  B Weber; U Nestler; W Ernst; H Rabenau; J Braner; A Birkenbach; E H Scheuermann; W Schoeppe; H W Doerr
Journal:  J Med Virol       Date:  1994-06       Impact factor: 2.327

9.  Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction.

Authors:  D Zipeto; F Baldanti; D Zella; M Furione; A Cavicchini; G Milanesi; G Gerna
Journal:  J Virol Methods       Date:  1993-09       Impact factor: 2.014

10.  Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Authors:  P M Holland; R D Abramson; R Watson; D H Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205
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  43 in total

Review 1.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR.

Authors:  Michael Boeckh; MeeiLi Huang; James Ferrenberg; Terry Stevens-Ayers; Laurence Stensland; W Garrett Nichols; Lawrence Corey
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

3.  Valnoctamide Inhibits Cytomegalovirus Infection in Developing Brain and Attenuates Neurobehavioral Dysfunctions and Brain Abnormalities.

Authors:  Sara Ornaghi; Lawrence S Hsieh; Angélique Bordey; Patrizia Vergani; Michael J Paidas; Anthony N van den Pol
Journal:  J Neurosci       Date:  2017-06-19       Impact factor: 6.167

4.  Cytomegalovirus infection in patients with ulcerative colitis diagnosed by quantitative real-time PCR analysis.

Authors:  Tadayuki Kou; Hiroshi Nakase; Hiroyuki Tamaki; Toyoichiro Kudo; Akiyoshi Nishio; Tsutomu Chiba
Journal:  Dig Dis Sci       Date:  2006-06       Impact factor: 3.199

Review 5.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

6.  Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.

Authors:  Martina Lengerova; Zdenek Racil; Pavlina Volfova; Jana Lochmanova; Jitka Berkovcova; Dana Dvorakova; Jiri Vorlicek; Jiri Mayer
Journal:  J Clin Microbiol       Date:  2007-01-17       Impact factor: 5.948

7.  Detection of cytomegalovirus in whole blood using three different real-time PCR chemistries.

Authors:  Erica Vincent; Zhengming Gu; Markus Morgenstern; Candace Gibson; Jianmin Pan; Randall T Hayden
Journal:  J Mol Diagn       Date:  2008-12-12       Impact factor: 5.568

8.  Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication.

Authors:  Blair L Strang; Steeve Boulant; Donald M Coen
Journal:  J Virol       Date:  2009-12-09       Impact factor: 5.103

9.  Monitoring cytomegalovirus infection in adult and pediatric bone marrow transplant recipients by a real-time PCR assay performed with blood plasma.

Authors:  Marianne Leruez-Ville; Marie Ouachée; Richard Delarue; Anne-Sophie Sauget; Stéphane Blanche; Agnès Buzyn; Christine Rouzioux
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

10.  Validation of clinical application of cytomegalovirus plasma DNA load measurement and definition of treatment criteria by analysis of correlation to antigen detection.

Authors:  Jayant S Kalpoe; Aloys C M Kroes; Menno D de Jong; Janke Schinkel; Caroline S de Brouwer; Matthias F C Beersma; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948
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