Literature DB >> 10878073

Detection of human cytomegalovirus DNA by real-time quantitative PCR.

A Nitsche1, N Steuer, C A Schmidt, O Landt, H Ellerbrok, G Pauli, W Siegert.   

Abstract

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.

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Year:  2000        PMID： 10878073      PMCID： PMC87012     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

Review 1.  Cytomegalovirus infection after organ transplantation: an update with special emphasis on renal transplantation.

Authors:  W J van Son; T H The
Journal:  Transpl Int       Date:  1989-10       Impact factor: 3.782

2.  Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA.

Authors:  A Nitsche; N Steuer; C A Schmidt; O Landt; W Siegert
Journal:  Clin Chem       Date:  1999-11       Impact factor: 8.327

3.  Demonstration of cytomegalovirus after bone marrow transplantation by polymerase chain reaction, virus culture and antigen detection in buffy coat leukocytes.

Authors:  C A Schmidt; H Oettle; F Wilborn; J Jessen; H Timm; R Schwerdtfeger; J Oertel; W Siegert
Journal:  Bone Marrow Transplant       Date:  1994-01       Impact factor: 5.483

Review 4.  Cytomegalovirus infection after liver transplantation: clinical manifestations and strategies for prevention.

Authors:  J S Dummer
Journal:  Rev Infect Dis       Date:  1990 Sep-Oct

5.  Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients.

Authors:  T C Roberts; D C Brennan; R S Buller; M Gaudreault-Keener; M A Schnitzler; K E Sternhell; K A Garlock; G G Singer; G A Storch
Journal:  J Infect Dis       Date:  1998-09       Impact factor: 5.226

6.  A randomized, controlled trial of prophylactic ganciclovir for cytomegalovirus pulmonary infection in recipients of allogeneic bone marrow transplants; The City of Hope-Stanford-Syntex CMV Study Group.

Authors:  G M Schmidt; D A Horak; J C Niland; S R Duncan; S J Forman; J A Zaia
Journal:  N Engl J Med       Date:  1991-04-11       Impact factor: 91.245

7.  Treatment of invasive cytomegalovirus disease in solid organ transplant patients with ganciclovir.

Authors:  D L Dunn; J L Mayoral; K J Gillingham; C M Loeffler; K L Brayman; M A Kramer; A Erice; H H Balfour; C V Fletcher; R M Bolman
Journal:  Transplantation       Date:  1991-01       Impact factor: 4.939

8.  Early treatment with ganciclovir to prevent cytomegalovirus disease after allogeneic bone marrow transplantation.

Authors:  J M Goodrich; M Mori; C A Gleaves; C Du Mond; M Cays; D F Ebeling; W C Buhles; B DeArmond; J D Meyers
Journal:  N Engl J Med       Date:  1991-12-05       Impact factor: 91.245

9.  Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Authors:  P M Holland; R D Abramson; R Watson; D H Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

10.  Ganciclovir therapy of severe cytomegalovirus infections in solid-organ transplant recipients.

Authors:  M A Harbison; P C De Girolami; R L Jenkins; S M Hammer
Journal:  Transplantation       Date:  1988-07       Impact factor: 4.939
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  18 in total

Review 1.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis.

Authors:  Andreas Nitsche; Heinz Ellerbrok; Georg Pauli
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

Review 3.  Diagnosis of hepatitis a virus infection: a molecular approach.

Authors:  Omana V Nainan; Guoliang Xia; Gilberto Vaughan; Harold S Margolis
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

Review 4.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

5.  Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients.

Authors:  Concepción Gimeno; Carlos Solano; José C Latorre; Juan C Hernández-Boluda; María A Clari; María J Remigia; Santiago Furió; Marisa Calabuig; Nuria Tormo; David Navarro
Journal:  J Clin Microbiol       Date:  2008-08-27       Impact factor: 5.948

6.  Quantification of human cytomegalovirus DNA by real-time PCR.

Authors:  E Gault; Y Michel; A Dehée; C Belabani; J C Nicolas; A Garbarg-Chenon
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

7.  Detection of cytomegalovirus in whole blood using three different real-time PCR chemistries.

Authors:  Erica Vincent; Zhengming Gu; Markus Morgenstern; Candace Gibson; Jianmin Pan; Randall T Hayden
Journal:  J Mol Diagn       Date:  2008-12-12       Impact factor: 5.568

8.  Monitoring cytomegalovirus infection in adult and pediatric bone marrow transplant recipients by a real-time PCR assay performed with blood plasma.

Authors:  Marianne Leruez-Ville; Marie Ouachée; Richard Delarue; Anne-Sophie Sauget; Stéphane Blanche; Agnès Buzyn; Christine Rouzioux
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

9.  Detection and genotyping of varicella-zoster virus by TaqMan allelic discrimination real-time PCR.

Authors:  Paul A Campsall; Nicholas H C Au; Julie S Prendiville; David P Speert; Rusung Tan; Eva E Thomas
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

10.  Validation of clinical application of cytomegalovirus plasma DNA load measurement and definition of treatment criteria by analysis of correlation to antigen detection.

Authors:  Jayant S Kalpoe; Aloys C M Kroes; Menno D de Jong; Janke Schinkel; Caroline S de Brouwer; Matthias F C Beersma; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948
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