Literature DB >> 11158147

Quantitative detection of hepatitis C virus RNA by light cycler PCR and comparison with two different PCR assays.

M Schröter1, B Zöllner, P Schäfer, R Laufs, H H Feucht.   

Abstract

The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.

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Year:  2001        PMID： 11158147      PMCID： PMC87816          DOI： 10.1128/JCM.39.2.765-768.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Antibody response to core, envelope and nonstructural hepatitis C virus antigens: comparison of immunocompetent and immunosuppressed patients.

Authors:  A S Lok; D Chien; Q L Choo; T M Chan; E K Chiu; I K Cheng; M Houghton; G Kuo
Journal:  Hepatology       Date:  1993-09       Impact factor: 17.425

2.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

3.  The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

Authors:  C T Wittwer; K M Ririe; R V Andrew; D A David; R A Gundry; U J Balis
Journal:  Biotechniques       Date:  1997-01       Impact factor: 1.993

4.  Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.

Authors:  P A Cane; P Cook; D Ratcliffe; D Mutimer; D Pillay
Journal:  Antimicrob Agents Chemother       Date:  1999-07       Impact factor: 5.191

5.  Quantitative PCR: theoretical considerations with practical implications.

Authors:  L Raeymaekers
Journal:  Anal Biochem       Date:  1993-11-01       Impact factor: 3.365

6.  Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays.

Authors:  G Haberhausen; J Pinsl; C C Kuhn; C Markert-Hahn
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948

7.  Tear fluid of hepatitis C virus carriers could be infectious.

Authors:  H H Feucht; B Zöllner; M Schröter; H Altrogge; R Laufs
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

8.  High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA.

Authors:  M Martell; J Gómez; J I Esteban; S Sauleda; J Quer; B Cabot; R Esteban; J Guardia
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

9.  High percentage of seronegative HCV infections in hemodialysis patients: the need for PCR.

Authors:  M Schröter; H H Feucht; P Schäfer; B Zöllner; R Laufs
Journal:  Intervirology       Date:  1997       Impact factor: 1.763

10.  Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank setting.

Authors:  W K Roth; M Weber; E Seifried
Journal:  Lancet       Date:  1999-01-30       Impact factor: 79.321
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  10 in total

1.  LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system.

Authors:  S J Read; J L Mitchell; C G Fink
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Genotyping of hepatitis C virus types 1, 2, 3, and 4 by a one-step LightCycler method using three different pairs of hybridization probes.

Authors:  Matthias Schröter; Bernhard Zöllner; Peter Schäfer; Olfert Landt; Ulrich Lass; Rainer Laufs; Heinz-Hubert Feucht
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

4.  A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle.

Authors:  Changhyun Roh; Ho-Young Lee; Sang-Eun Kim; Sung-Kee Jo
Journal:  Int J Nanomedicine       Date:  2010-05-13

5.  External quality assessment program for qualitative and quantitative detection of hepatitis C virus RNA in diagnostic virology.

Authors:  Jurjen Schirm; Anton M van Loon; Elizabeth Valentine-Thon; Paul E Klapper; Jim Reid; Graham M Cleator
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

6.  Variable ratio of hepatitis C virus RNA to viral core antigen in patient sera.

Authors:  Christian G Schüttler; Christine Thomas; Thomas Discher; Georg Friese; Jürgen Lohmeyer; Ralph Schuster; Stephan Schaefer; Wolfram H Gerlich
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

7.  Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA.

Authors:  Linda Cook; Ka-Wing Ng; Arthur Bagabag; Lawrence Corey; Keith R Jerome
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

8.  Hepatitis C Virus (HCV) Vertical Transmission in 12-Month-Old Infants Born to HCV-Infected Women and Assessment of Maternal Risk Factors.

Authors:  Ravi Jhaveri; Mohamed Hashem; Samer S El-Kamary; Doa'a A Saleh; Sahar A Sharaf; Fatma El-Mougy; Lobna Abdelsalam; Mohamed Ehab; Hesham El-Ghazaly
Journal:  Open Forum Infect Dis       Date:  2015-06-26       Impact factor: 3.835

9.  Relevance of reactivity in commercially available hepatitis C virus antibody assays.

Authors:  S Polywka; M Schröter; H H Feucht; B Zöllner; R Laufs
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

10.  Spontaneous Viral Load Decline and Subsequent Clearance of Chronic Hepatitis C Virus in Postpartum Women Correlates With Favorable Interleukin-28B Gene Allele.

Authors:  Mohamed Hashem; Ravi Jhaveri; Doa'a A Saleh; Sahar A Sharaf; Fatma El-Mougy; Lobna Abdelsalam; Michelle D Shardell; Hesham El-Ghazaly; Samer S El-Kamary
Journal:  Clin Infect Dis       Date:  2017-09-15       Impact factor: 9.079
  10 in total

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