Literature DB >> 11139214

Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA.

S Carnevale1, M I Rodríguez, G Santillán, J H Labbé, M G Cabrera, E J Bellegarde, J N Velásquez, J E Trgovcic, E A Guarnera.   

Abstract

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.

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Year:  2001        PMID： 11139214      PMCID： PMC96029          DOI： 10.1128/CDLI.8.1.174-177.2001

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  23 in total

1.  Use of the Falcon assay screening test--enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectrotransfer blot (EITB) to determine the prevalence of human fascioliasis in the Bolivian Altiplano.

Authors:  G V Hillyer; M Soler de Galanes; J Rodriguez-Perez; J Bjorland; M Silva de Lagrava; S Ramirez Guzman; R T Bryan
Journal:  Am J Trop Med Hyg       Date:  1992-05       Impact factor: 2.345

2.  Fasciola hepatica: immunoprecipitation analysis of biosynthetically labelled antigens using sera from infected sheep.

Authors:  J L Sexton; A R Milner; N J Campbell
Journal:  Parasite Immunol       Date:  1991-01       Impact factor: 2.280

3.  Immunodiagnosis of human fascioliasis by enzyme-linked immunosorbent assay using excretory-secretory products.

Authors:  A M Espino; B E Duménigo; R Fernández; C M Finlay
Journal:  Am J Trop Med Hyg       Date:  1987-11       Impact factor: 2.345

4.  Comparison of counterelectrophoresis the enzyme-linked immunosorbent assay, and Kato fecal examination for the diagnosis of fascioliasis in infected mice and rabbits.

Authors:  D M Levine; G V Hillyer; S I Flores
Journal:  Am J Trop Med Hyg       Date:  1980-07       Impact factor: 2.345

5.  An outbreak of acute fascioliasis among Aymara Indians in the Bolivian Altiplano.

Authors:  J Bjorland; R T Bryan; W Strauss; G V Hillyer; J B McAuley
Journal:  Clin Infect Dis       Date:  1995-11       Impact factor: 9.079

6.  Sandwich enzyme-linked immunosorbent assay for detection of excretory secretory antigens in humans with fascioliasis.

Authors:  A M Espino; C M Finlay
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

7.  [Fascioliasis in populations of rural areas with high prevalence of animal infection].

Authors:  W Apt; X Aguilera; F Vega; I Zulantay; C Retamal; P Apt; J Sandoval
Journal:  Rev Med Chil       Date:  1992-06       Impact factor: 0.553

8.  A simple and economic slide micro-immunoenzymatic (micro-SIA) test for epidemiological studies of toxoplasmosis.

Authors:  M Rosenzvit; S Angel; L Alvarez; V Mellare; J Blanco; V Pszenny; J C Garberi
Journal:  Mem Inst Oswaldo Cruz       Date:  1994 Jan-Mar       Impact factor: 2.743

Review 9.  Fascioliasis in developed countries: a review of classic and aberrant forms of the disease.

Authors:  R Arjona; J A Riancho; J M Aguado; R Salesa; J González-Macías
Journal:  Medicine (Baltimore)       Date:  1995-01       Impact factor: 1.889

10.  Antigenic components of excretory-secretory products of adult Fasciola hepatica recognized in human infections.

Authors:  M L Sampaio-Silva; J M Da Costa; A M Da Costa; M A Pires; S A Lopes; A M Castro; L Monjour
Journal:  Am J Trop Med Hyg       Date:  1996-02       Impact factor: 2.345
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  25 in total

1.  Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica.

Authors:  José F Gaudier; Kimberly Cabán-Hernández; Antonio Osuna; Ana M Espino
Journal:  Clin Vaccine Immunol       Date:  2012-01-25

2.  Does Fasciola hepatica infection modify the response of acute hepatitis C virus infection to IFN-alpha treatment?

Authors:  Mehmet Sahin; Mehmet Isler; Altug Senol; Mustafa Demirci; Zeynep Dilek Aydin
Journal:  World J Gastroenterol       Date:  2005-12-28       Impact factor: 5.742

3.  Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.

Authors:  Kimberly Cabán-Hernández; José F Gaudier; Caleb Ruiz-Jiménez; Ana M Espino
Journal:  J Clin Microbiol       Date:  2013-12-18       Impact factor: 5.948

4.  Evaluation of a 14.5 kDa-Fasciola gigantica fatty acid binding protein as a diagnostic antigen for human fascioliasis.

Authors:  Gamal Allam; Ibrahim R Bauomy; Zeinab M Hemyeda; Thabet F Sakran
Journal:  Parasitol Res       Date:  2011-11-24       Impact factor: 2.289

5.  Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings.

Authors:  M Gonzalo-Orden; L Millán; M Alvarez; S Sánchez-Campos; R Jiménez; J González-Gallego; M J Tuñón
Journal:  Parasitol Res       Date:  2003-04-17       Impact factor: 2.289

6.  Fasciola hepatica saposin-like protein-2-based ELISA for the serodiagnosis of chronic human fascioliasis.

Authors:  Olgary Figueroa-Santiago; Bonnibel Delgado; Ana M Espino
Journal:  Diagn Microbiol Infect Dis       Date:  2011-07       Impact factor: 2.803

7.  Direct, immunological and molecular techniques for a fasciolosis survey in a rural area of San Luis, Argentina.

Authors:  Silvana Carnevale; Marta Graciela Cabrera; Marcela Alejandra Cucher; Cecilia Alicia di Risio; Jorge Bruno Malandrini; Laura Kamenetzky; Marcio Alazraqui; Cristina Beatriz Etchart; María Laura Pantano; Jorge Néstor Velásquez
Journal:  J Parasit Dis       Date:  2012-09-28

8.  Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens.

Authors:  Kobra Mokhtarian; Lame Akhlaghi; Ahmad Reza Meamar; Elham Razmjou; Kourosh Manouchehri Naeini; Samaneh Gholami; Masoomeh Najafi Samei; Reza Falak
Journal:  Parasitol Res       Date:  2016-04-30       Impact factor: 2.289

9.  Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens.

Authors:  Seobo Sim; Gab-Man Park; Tai-Soon Yong
Journal:  Parasitol Res       Date:  2003-09-16       Impact factor: 2.289

10.  Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections.

Authors:  A Marcilla; J E De la Rubia; J Sotillo; D Bernal; C Carmona; Z Villavicencio; D Acosta; J Tort; F J Bornay; J G Esteban; R Toledo
Journal:  Clin Vaccine Immunol       Date:  2007-11-14
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