Literature DB >> 14574567

Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens.

Seobo Sim1, Gab-Man Park, Tai-Soon Yong.   

Abstract

Clonorchis sinensis excretory-secretory (ES) antigens were separated by gradient SDS-PAGE and the antigenic protein isolated at 21 kDa was injected into rats. A C. sinensis cDNA library was then immunoscreened with sera from the 21-kDa antigenic protein-immunized rats. The selected genes, which were named C. sinensis myoglobin, contained a single open reading frame of 450 base pairs encoding 150 amino acids. A single hybridized band of 0.57 kb and two to four hybridized bands were detected by Northern and Southern blotting. Purified recombinant C. sinensis myoglobin was recognized by clonorchiasic rabbit sera (50%) and clonorchiasic human sera (25%). An indirect immunofluorescence assay showed that C. sinensis myoglobin is distributed over the whole body of the adult worm. It is believed that the abundance of C. sinensis myoglobin plays an important role as an oxygen reservoir under anaerobic conditions.

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Year:  2003        PMID： 14574567     DOI： 10.1007/s00436-003-0869-7

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  21 in total

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Journal:  Hepatogastroenterology       Date:  2000 Jul-Aug

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Authors:  L Cervi; G Rossi; D T Masih
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4.  Structural and functional comparison of trematode haemoglobins.

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Review 5.  Liver and intestinal flukes.

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Journal:  Gastroenterol Clin North Am       Date:  1996-09       Impact factor: 3.806

Review 6.  Clonorchiasis in Korea.

Authors:  H J Rim
Journal:  Kisaengchunghak Chapchi       Date:  1990-12

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Authors:  A K Rashid; M L Van Hauwaert; M Haque; A H Siddiqi; I Lasters; M De Maeyer; N Griffon; M C Marden; S Dewilde; J Clauwaert; S N Vinogradov; L Moens
Journal:  J Biol Chem       Date:  1997-01-31       Impact factor: 5.157

8.  A Clonorchis sinensis-specific antigen that detects active human clonorchiasis.

Authors:  S I Kim
Journal:  Korean J Parasitol       Date:  1998-03       Impact factor: 1.341

9.  Immunodiagnosis of clonorchiasis using a recombinant antigen.

Authors:  T S Yong; H J Yang; S J Park; Y K Kim; D H Lee; S M Lee
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10.  Trematode hemoglobins show exceptionally high oxygen affinity.

Authors:  L Kiger; A K Rashid; N Griffon; M Haque; L Moens; Q H Gibson; C Poyart; M C Marden
Journal:  Biophys J       Date:  1998-08       Impact factor: 4.033
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  7 in total

1.  Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis.

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Journal:  Parasitol Res       Date:  2007-02-23       Impact factor: 2.289

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3.  Organ-specific antigens of Clonorchis sinensis.

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4.  Immunomodulatory action of excretory-secretory products of Angiostrongylus cantonensis in a mouse tumour model.

Authors:  Yajun Lu; Yuxiao Yang; Siqi Yang; Qianfeng Xia
Journal:  Parasitol Res       Date:  2020-09-08       Impact factor: 2.289

5.  The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study.

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Journal:  Protein Sci       Date:  2008-07-11       Impact factor: 6.725

6.  The carcinogenic liver fluke, Clonorchis sinensis: new assembly, reannotation and analysis of the genome and characterization of tissue transcriptomes.

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Journal:  PLoS One       Date:  2013-01-30       Impact factor: 3.240

7.  Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini.

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Journal:  BMC Genomics       Date:  2007-06-22       Impact factor: 3.969
  7 in total

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