BACKGROUND: Leptin is an important regulator of food intake and energy expenditure. Initially it was thought to be expressed exclusively in and secreted by adipocytes. Recently, leptin expression was also noted in other tissues, including rat gastric mucosa. Information on leptin and leptin receptor expression in the human stomach is lacking. AIM: To investigate expression of leptin and its corresponding receptors in human gastric epithelial cells. METHODS: Fundic and antral gastric mucosal biopsies, primary cultures of human gastric epithelial cells, and the human gastric cancer cell line AGS were screened for expression of leptin and different leptin receptor isoform mRNA by reverse transcriptase-polymerase chain reaction. Immunohistochemistry was performed for localisation of leptin and leptin receptor proteins in gastric mucosa. RESULTS: mRNA of leptin and its four receptor isoforms (huOB-R, long receptor isoform; huB219.1-3, short receptor isoforms) was detected in gastric mucosal biopsies, cultured human gastric epithelial cells, and gastric cancer cells. Immunohistochemistry demonstrated that chief as well as parietal cells were reactive to leptin and leptin receptors. CONCLUSIONS: Leptin and leptin receptors are expressed in human gastric mucosa. These findings suggest a paracrine and/or autocrine effect of leptin on gastric epithelial cell function.
BACKGROUND: Leptin is an important regulator of food intake and energy expenditure. Initially it was thought to be expressed exclusively in and secreted by adipocytes. Recently, leptin expression was also noted in other tissues, including rat gastric mucosa. Information on leptin and leptin receptor expression in the human stomach is lacking. AIM: To investigate expression of leptin and its corresponding receptors in human gastric epithelial cells. METHODS: Fundic and antral gastric mucosal biopsies, primary cultures of human gastric epithelial cells, and the human gastric cancer cell line AGS were screened for expression of leptin and different leptin receptor isoform mRNA by reverse transcriptase-polymerase chain reaction. Immunohistochemistry was performed for localisation of leptin and leptin receptor proteins in gastric mucosa. RESULTS: mRNA of leptin and its four receptor isoforms (huOB-R, long receptor isoform; huB219.1-3, short receptor isoforms) was detected in gastric mucosal biopsies, cultured human gastric epithelial cells, and gastric cancer cells. Immunohistochemistry demonstrated that chief as well as parietal cells were reactive to leptin and leptin receptors. CONCLUSIONS: Leptin and leptin receptors are expressed in human gastric mucosa. These findings suggest a paracrine and/or autocrine effect of leptin on gastric epithelial cell function.
Authors: T Brzozowski; P C Konturek; S J Konturek; R Pajdo; A Duda; P Pierzchalski; W Bielański; E G Hahn Journal: Eur J Pharmacol Date: 1999-06-18 Impact factor: 4.432
Authors: L A Tartaglia; M Dembski; X Weng; N Deng; J Culpepper; R Devos; G J Richards; L A Campfield; F T Clark; J Deeds; C Muir; S Sanker; A Moriarty; K J Moore; J S Smutko; G G Mays; E A Wool; C A Monroe; R I Tepper Journal: Cell Date: 1995-12-29 Impact factor: 41.582
Authors: A Larsen; T Davis; B M Curtis; S Gimpel; J E Sims; D Cosman; L Park; E Sorensen; C J March; C A Smith Journal: J Exp Med Date: 1990-12-01 Impact factor: 14.307
Authors: D P Gearing; C J Thut; T VandeBos; S D Gimpel; P B Delaney; J King; V Price; D Cosman; M P Beckmann Journal: EMBO J Date: 1991-10 Impact factor: 11.598
Authors: Juanita H J Vernooy; Niki D J Ubags; Guy G Brusselle; Jan Tavernier; Benjamin T Suratt; Guy F Joos; Emiel F M Wouters; Ken R Bracke Journal: Pulm Pharmacol Ther Date: 2013-03-27 Impact factor: 3.410
Authors: Zhiying Li; Giovanni Ceccarini; Michael Eisenstein; Keith Tan; Jeffrey Michael Friedman Journal: Mol Metab Date: 2013-08-04 Impact factor: 7.422
Authors: Clémence D Côté; Melika Zadeh-Tahmasebi; Brittany A Rasmussen; Frank A Duca; Tony K T Lam Journal: J Biol Chem Date: 2014-02-27 Impact factor: 5.157