Literature DB >> 10966112

Initiation of protein synthesis from the A site of the ribosome.

J E Wilson1, T V Pestova, C U Hellen, P Sarnow.   

Abstract

Positioning of the translation initiation complex on mRNAs requires interaction between the anticodon of initiator Met-tRNA, associated with eIF2-GTP and 40S ribosomal subunit, and the cognate start codon of the mRNA. We show that an internal ribosome entry site located in the genome of cricket paralysis virus can form 80S ribosomes without initiator Met-tRNA, eIF2, or GTP hydrolysis, with a CCU triplet in the ribosomal P site and a GCU triplet in the A site. P-site mutagenesis revealed that the P site was not decoded, and protein sequence analysis showed that translation initiates at the triplet in the A site. Translational initiation from the A site of the ribosome suggests that the repertoire of translated open reading frames in eukaryotic mRNAs may be greater than anticipated.

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Year:  2000        PMID： 10966112     DOI： 10.1016/s0092-8674(00)00055-6

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  203 in total

1.  Design of multistable RNA molecules.

Authors:  C Flamm; I L Hofacker; S Maurer-Stroh; P F Stadler; M Zehl
Journal:  RNA       Date:  2001-02       Impact factor: 4.942

Review 2.  New ways of initiating translation in eukaryotes?

Authors:  M Kozak
Journal:  Mol Cell Biol       Date:  2001-03       Impact factor: 4.272

3.  New ways of initiating translation in eukaryotes.

Authors:  R Schneider; V I Agol; R Andino; F Bayard; D R Cavener; S A Chappell; J J Chen; J L Darlix; A Dasgupta; O Donzé; R Duncan; O Elroy-Stein; P J Farabaugh; W Filipowicz; M Gale; L Gehrke; E Goldman; Y Groner; J B Harford; M Hatzglou; B He; C U Hellen; M W Hentze; J Hershey; P Hershey; T Hohn; M Holcik; C P Hunter; K Igarashi; R Jackson; R Jagus; L S Jefferson; B Joshi; R Kaempfer; M Katze; R J Kaufman; M Kiledjian; S R Kimball; A Kimchi; K Kirkegaard; A E Koromilas; R M Krug; V Kruys; B J Lamphear; S Lemon; R E Lloyd; L E Maquat; E Martinez-Salas; M B Mathews; V P Mauro; S Miyamoto; I Mohr; D R Morris; E G Moss; N Nakashima; A Palmenberg; N T Parkin; T Pe'ery; J Pelletier; S Peltz; T V Pestova; E V Pilipenko; A C Prats; V Racaniello; G S Read; R E Rhoads; J D Richter; R Rivera-Pomar; T Rouault; A Sachs; P Sarnow; G C Scheper; L Schiff; D R Schoenberg; B L Semler; A Siddiqui; T Skern; N Sonenberg; W Sossin; N Standart; S M Tahara; A A Thomas; J J Toulmé; J Wilusz; E Wimmer; G Witherell; M Wormington
Journal:  Mol Cell Biol       Date:  2001-12       Impact factor: 4.272

4.  Unleashing yeast genetics on a factor-independent mechanism of internal translation initiation.

Authors:  A G Hinnebusch
Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-06       Impact factor: 11.205

5.  Computational modeling of eukaryotic mRNA turnover.

Authors:  D Cao; R Parker
Journal:  RNA       Date:  2001-09       Impact factor: 4.942

Review 6.  Irresistible IRES. Attracting the translation machinery to internal ribosome entry sites.

Authors:  S Vagner; B Galy; S Pyronnet
Journal:  EMBO Rep       Date:  2001-10       Impact factor: 8.807

Review 7.  Viral internal ribosome entry site elements: novel ribosome-RNA complexes and roles in viral pathogenesis.

Authors:  Peter Sarnow
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

8.  Structural elements in the internal ribosome entry site of Plautia stali intestine virus responsible for binding with ribosomes.

Authors:  Takashi Nishiyama; Hiroshi Yamamoto; Norihiro Shibuya; Yoshinori Hatakeyama; Akira Hachimori; Toshio Uchiumi; Nobuhiko Nakashima
Journal:  Nucleic Acids Res       Date:  2003-05-01       Impact factor: 16.971

Review 9.  Control of eukaryotic protein synthesis by upstream open reading frames in the 5'-untranslated region of an mRNA.

Authors:  Hedda A Meijer; Adri A M Thomas
Journal:  Biochem J       Date:  2002-10-01       Impact factor: 3.857

10.  Severe acute respiratory syndrome coronavirus protein nsp1 is a novel eukaryotic translation inhibitor that represses multiple steps of translation initiation.

Authors:  Kumari G Lokugamage; Krishna Narayanan; Cheng Huang; Shinji Makino
Journal:  J Virol       Date:  2012-10-03       Impact factor: 5.103
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