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Literature DB >> 1096084

An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.

Abstract

An improved method for the rapid separation of aminoacyl-tRNA from tRNA by chromatography on dihydroxyboryl-substituted cellulose has been developed. The method relies on the selective binding of unacylated tRNA to the cell cellulose support containing dihydroxyboryl groups. This binding is the result of complex formation between the cis-diol group of the 3'-terminal ribose in tRNA and the dihydroxyboryl groups immobilized on the resin. Aminoacyl-tRNA cannot undergo borate complex formation and is not retained on the resin. The separation is carried out at near neutral pH values ensuring stability of the aminoacyl ester linkage. The aminoacyl-tRNAs are obtained in very high purity. Aminoacyl-tRNA species containing the modified nucleoside Q are also retained on dihydroxyboryl cellulose. Conditions for isolating all Q base containing tRNA species from unfractionated tRNA are described.

Entities: Chemical

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Year: 1975 PMID： 1096084 PMCID： PMC343472 DOI： 10.1093/nar/2.6.853

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. The nucleotide sequence of a serine tRNA from Escherichia coli.

Authors: H Ishikura; Y Yamada; S Nishimura
Journal: FEBS Lett Date: 1971-07-15 Impact factor: 4.124

2. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

Authors: Y Furuichi; S Muthukrishnan; A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

3. Alterations in SVT2 cell transfer RNAs in response to cell density and serum type.

Authors: J R Katze
Journal: Biochim Biophys Acta Date: 1975-03-10

4. 3-(3-amino-3-carboxy-n-propyl)uridine. The structure of the nucleoside in Escherichia coli transfer ribonucleic acid that reacts with phenoxyacetoxysuccinimide.

Authors: S Friedman; H J Li; K Nakanishi; G Van Lear
Journal: Biochemistry Date: 1974-07-02 Impact factor: 3.162

5. Purification of chicken liver seryl transfer ribonucleic acid by complex formation with elongation factor EF-Tu:GTP. A general micromethod of aminoacyl transfer ribonucleic acid purification.

Authors: B J Klyde; M R Bernfield
Journal: Biochemistry Date: 1973-09-11 Impact factor: 3.162

6. Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid.

Authors: S Friedman
Journal: Biochemistry Date: 1972-08-29 Impact factor: 3.162

7. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.

Authors: B N White; G M Tener
Journal: J Mol Biol Date: 1973-03-15 Impact factor: 5.469

8. Studies on the interactions of nucleotides, polynucleotides, and nucleic acids with dihydroxyboryl-substituted celluloses.

Authors: M Rosenberg; J L Wiebers; P T Gilham
Journal: Biochemistry Date: 1972-09-12 Impact factor: 3.162

9. Synthesis of cellulose derivatives containing the dihydroxyboryl group and a study of their capacity to form specific complexes with sugars and nucleic acid components.

Authors: H L Weith; J L Wiebers; P T Gilham
Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

10. Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules.

Authors: M Rosenberg
Journal: Nucleic Acids Res Date: 1974-05 Impact factor: 16.971

30 in total

1. Efficiency of a self-aminoacylating ribozyme: effect of the length and base-composition of its 3' extension.

Authors: Jean Lehmann; Amy Reichel; Axel Buguin; Albert Libchaber
Journal: RNA Date: 2007-06-07 Impact factor: 4.942

2. In vivo aminoacylation of transfer ribonucleic acid in Bacillus subtilis and evidence for differential utilization of lysine-isoaccepting transfer ribonucleic acid species.

Authors: J Tockman; B S Vold
Journal: J Bacteriol Date: 1977-06 Impact factor: 3.490

3. Presence of 5'-terminal cap structures in virus-specific RNA from feline leukemia virus-infected cells.

Authors: A R Thomason; K H Friderici; L F Velicer; F Rottman
Journal: J Virol Date: 1978-05 Impact factor: 5.103

4. Chemical modification as a probe of conformational changes in transfer ribonucleic acid on aminoacylation.

Authors: M Lowdon; J P Goddard
Journal: Biochem J Date: 1978-06-01 Impact factor: 3.857

5. Localization of the 5' terminus of late SV40 mRNA.

Authors: G Haegeman; W Fiers
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

6. Isomeric aminoacyl-tRNAs are both bound by elongation factor Tu.

Authors: S M Hecht; K H Tan; A C Chinault; P Arcari
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

7. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. VI. Mutants with increased levels of glutaminyl-transfer ribonucleic acid synthetase and of glutamine transfer ribonucleic acid.

Authors: A Cheung; S Morgan; K B Low; D Söll
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

8. Chemical modification study of aminoacyl-tRNA conformation.

Authors: K Negishi; S Nishimura; F Harada; H Hayatsu
Journal: Nucleic Acids Res Date: 1979-03 Impact factor: 16.971

9. Separation of glycosylated haemoglobins using immobilized phenylboronic acid. Effect of ligand concentration, column operating conditions, and comparison with ion-exchange and isoelectric-focusing.

Authors: F A Middle; A Bannister; A J Bellingham; P D Dean
Journal: Biochem J Date: 1983-03-01 Impact factor: 3.857

10. Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography.

Authors: N Okada; N Shindo-Okada; S Nishimura
Journal: Nucleic Acids Res Date: 1977-02 Impact factor: 16.971