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Literature DB >> 190593

Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography.

Abstract

tRNAAsp from rabbit liver, rat liver and rat ascites hepatoma was readily isolated by concanavalin A-Sepharose (Con A-Sepharose) affinity column chromatography. tRNATyr from these sources was extensively purified by Ricinus communis lectin-Sepharose column chromatography. These results, together with the chromatographic behaviour of four tRNAs (tRNATyr, tRNAHis, tRNAAsn and tRNAAsp) on acetylated DBAE-cellulose column chromatography suggested that tRNAAsp contains a Q nucleoside species having a mannose moiety while tRNATyr contains Q nucleoside with galactose. The sugars attached in 4-position of cyclopentene diol in the Q molecule are therefore not present at random in the four tRNAs, but present only in each specific tRNA. This is the first case which shows that plant agglutinin interacts with nucleic Acid as well as polysaccharide and glycoproteins.

Entities: Chemical Disease Species

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Year: 1977 PMID： 190593 PMCID： PMC342442 DOI： 10.1093/nar/4.2.415

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.

Authors: H Kasai; Y Kuchino; K Nihei; S Nishimura
Journal: Nucleic Acids Res Date: 1975-10 Impact factor: 16.971

2. PROTEIN-CARBOHYDRATE INTERACTION. I. THE INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A.

Authors: I J GOLDSTEIN; C E HOLLERMAN; J M MERRICK
Journal: Biochim Biophys Acta Date: 1965-01-04

Review 3. The biochemistry of plant lectins (phytohemagglutinins).

Authors: H Lis; N Sharon
Journal: Annu Rev Biochem Date: 1973 Impact factor: 23.643

4. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.

Authors: M Simsek; J Ziegenmeyer; J Heckman; U L Rajbhandary
Journal: Proc Natl Acad Sci U S A Date: 1973-04 Impact factor: 11.205

5. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.

Authors: T Ikemura; J E Dahlberg
Journal: J Biol Chem Date: 1973-07-25 Impact factor: 5.157

6. 5 s RNA conformation. Studies of its partial T 1 ribonuclease digestion by gel electrophoresis and two-dimensional thin-layer chromatography.

Authors: A D Mirzabekov; B E Griffin
Journal: J Mol Biol Date: 1972-12-30 Impact factor: 5.469

7. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids.

Authors: F Harada; S Nishimura
Journal: Biochemistry Date: 1972-01-18 Impact factor: 3.162

8. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.

Authors: B N White; G M Tener
Journal: J Mol Biol Date: 1973-03-15 Impact factor: 5.469

9. An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.

Authors: T F McCutchan; P T Gilham; D Söll
Journal: Nucleic Acids Res Date: 1975-06 Impact factor: 16.971

10. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine.

Authors: H Kasai; Z Oashi; F Harada; S Nishimura; N J Oppenheimer; P F Crain; J G Liehr; D L von Minden; J A McCloskey
Journal: Biochemistry Date: 1975-09-23 Impact factor: 3.162

23 in total

1. Functionally impaired tRNA from ethionine treated rats as detected in injected Xenopus oocytes.

Authors: I Ginzburg; P Cornelis; D Giveon; U Z Littauer
Journal: Nucleic Acids Res Date: 1979-02 Impact factor: 16.971

2. Transfer RNA-like structure of the human Alu family: implications of its generation mechanism and possible functions.

Authors: N Okada
Journal: J Mol Evol Date: 1990-12 Impact factor: 2.395

3. Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.

Authors: J R Katze
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

Review 4. Biosynthesis of pyrrolopyrimidines.

Authors: Reid M McCarty; Vahe Bandarian
Journal: Bioorg Chem Date: 2012-01-31 Impact factor: 5.275

Review 5. Structure and function of preQ₁ riboswitches.

Authors: Catherine D Eichhorn; Mijeong Kang; Juli Feigon
Journal: Biochim Biophys Acta Date: 2014-05-04

6. Enzymatic synthesis of Q nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver.

Authors: N Okada; S Nishimura
Journal: Nucleic Acids Res Date: 1977-08 Impact factor: 16.971

Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography.

1. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.

2. PROTEIN-CARBOHYDRATE INTERACTION. I. THE INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A.

Review 3. The biochemistry of plant lectins (phytohemagglutinins).

4. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.

5. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.

6. 5 s RNA conformation. Studies of its partial T 1 ribonuclease digestion by gel electrophoresis and two-dimensional thin-layer chromatography.

7. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids.

8. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.

9. An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.

10. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine.

1. Functionally impaired tRNA from ethionine treated rats as detected in injected Xenopus oocytes.

2. Transfer RNA-like structure of the human Alu family: implications of its generation mechanism and possible functions.

3. Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.

Review 4. Biosynthesis of pyrrolopyrimidines.

Review 5. Structure and function of preQ₁ riboswitches.

6. Enzymatic synthesis of Q nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver.

7. Comparison of rat liver and Walker 256 carcinosarcoma tRNAs.

8. The role of queuine in the aminoacylation of mammalian aspartate transfer RNAs.

9. Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme.

10. Chromatographic behavior of several mammalian tRNAs on acylated dihydroxyl-borate cellulose and Aminex A-28.

Review 3. The biochemistry of plant lectins (phytohemagglutinins).

Review 4. Biosynthesis of pyrrolopyrimidines.

Review 5. Structure and function of preQ1 riboswitches.

Review 5. Structure and function of preQ₁ riboswitches.