Literature DB >> 6870791

Separation of glycosylated haemoglobins using immobilized phenylboronic acid. Effect of ligand concentration, column operating conditions, and comparison with ion-exchange and isoelectric-focusing.

F A Middle, A Bannister, A J Bellingham, P D Dean.   

Abstract

Haemoglobins from diabetic and non-diabetic individuals have been separated by affinity chromatography using immobilized phenylboronate, which interacts specifically with diol-containing compounds such as glycosylated haemoglobin. The effects of ligand concentration, flow rate, column geometry, preincubation of sample, buffer composition and temperature have been investigated. Significant correlation was found between results from affinity-chromatography and ion-exchange and isoelectric-focusing methods. Isoelectric-focusing of the haemoglobin fractions obtained from affinity chromatography indicate that, in addition to haemoglobin A1c, some haemoglobin A is also bound to immobilized phenylboronic acid. Assays of haemolysates obtained from red blood cells incubated in glucose solutions suggest that unstable pre-(haemoglobin A1c) does not interfere. The assay is not affected by the presence of haemoglobin F.

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Year:  1983        PMID： 6870791      PMCID： PMC1154156          DOI： 10.1042/bj2090771

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

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Authors:  R Flückiger; K H Winterhalter
Journal:  FEBS Lett       Date:  1976-12-01       Impact factor: 4.124

2.  Glycosylated hemoglobins: increased glycosylation of hemoglobin A in diabetic patients.

Authors:  K H Gabbay; J M Sosenko; G A Banuchi; M J Mininsohn; R Flückiger
Journal:  Diabetes       Date:  1979-04       Impact factor: 9.461

3.  Structural heterogeneity of human hemoglobin A due to nonenzymatic glycosylation.

Authors:  H F Bunn; R Shapiro; M McManus; L Garrick; M J McDonald; P M Gallop; K H Gabbay
Journal:  J Biol Chem       Date:  1979-05-25       Impact factor: 5.157

4.  Studies on the interactions of nucleotides, polynucleotides, and nucleic acids with dihydroxyboryl-substituted celluloses.

Authors:  M Rosenberg; J L Wiebers; P T Gilham
Journal:  Biochemistry       Date:  1972-09-12       Impact factor: 3.162

5.  Synthesis of cellulose derivatives containing the dihydroxyboryl group and a study of their capacity to form specific complexes with sugars and nucleic acid components.

Authors:  H L Weith; J L Wiebers; P T Gilham
Journal:  Biochemistry       Date:  1970-10-27       Impact factor: 3.162

6.  Hemoglobin components in patients with diabetes mellitus.

Authors:  L A Trivelli; H M Ranney; H T Lai
Journal:  N Engl J Med       Date:  1971-02-18       Impact factor: 91.245

7.  An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.

Authors:  T F McCutchan; P T Gilham; D Söll
Journal:  Nucleic Acids Res       Date:  1975-06       Impact factor: 16.971

8.  Sites of nonenzymatic glycosylation of human hemoglobin A.

Authors:  R Shapiro; M J McManus; C Zalut; H F Bunn
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

9.  Increased glycosylation of serum albumin in diabetes mellitus.

Authors:  R Dolhofer; O H Wieland
Journal:  Diabetes       Date:  1980-06       Impact factor: 9.461

10.  Glycosylated minor components of human adult hemoglobin. Purification, identification, and partial structural analysis.

Authors:  M J McDonald; R Shapiro; M Bleichman; J Solway; H F Bunn
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157
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  6 in total

Review 1.  Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments.

Authors:  David S Hage; Jeanethe A Anguizola; Cong Bi; Rong Li; Ryan Matsuda; Efthimia Papastavros; Erika Pfaunmiller; John Vargas; Xiwei Zheng
Journal:  J Pharm Biomed Anal       Date:  2012-01-14       Impact factor: 3.935

2.  Insulin, glucose and glycated hemoglobin in Alzheimer's and vascular dementia with and without superimposed Type II diabetes mellitus condition.

Authors:  R O Domínguez; E R Marschoff; E M Guareschi; M G Repetto; A L Famulari; M A Pagano; J A Serra
Journal:  J Neural Transm (Vienna)       Date:  2007-08-30       Impact factor: 3.575

3.  Purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose.

Authors:  S J Cartwright; S G Waley
Journal:  Biochem J       Date:  1984-07-15       Impact factor: 3.857

4.  Fructosamine is a useful indicator of hyperglycaemia and glucose control in clinical and epidemiological studies--cross-sectional and longitudinal experience from the AMORIS cohort.

Authors:  Håkan Malmström; Göran Walldius; Valdemar Grill; Ingmar Jungner; Soffia Gudbjörnsdottir; Niklas Hammar
Journal:  PLoS One       Date:  2014-10-29       Impact factor: 3.240

5.  Click reactions and boronic acids: applications, issues, and potential solutions.

Authors:  Chaofeng Dai; Yunfeng Cheng; Jianmei Cui; Binghe Wang
Journal:  Molecules       Date:  2010-08-23       Impact factor: 4.411

6.  Glycation of Host Proteins Increases Pathogenic Potential of Porphyromonas gingivalis.

Authors:  Michał Śmiga; John W Smalley; Paulina Ślęzak; Jason L Brown; Klaudia Siemińska; Rosalind E Jenkins; Edwin A Yates; Teresa Olczak
Journal:  Int J Mol Sci       Date:  2021-11-08       Impact factor: 5.923
  6 in total

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