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Literature DB >> 10934534

Time-resolved fluoroimmunoassay.

Abstract

A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time-resolved fluorescence in a home-made enhancement solution become feasible.

Entities: Chemical

Mesh：

Year: 1999 PMID： 10934534 DOI： 10.1385/MB:13:3:215

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

14 in total

1. A new fluorescence enhancement solution for europium-based time-resolved fluoroimmunoassays.

Authors: P Degan; A Abbondandolo; G Montagnoli
Journal: J Biolumin Chemilumin Date: 1990 Jul-Sep

2. Time resolved fluoroimmunoassay of 7-methyl-2'-deoxyguanosine imidazole (ring open).

Authors: P Degan; R Montesano; A Abbondandolo; G Montagnoli
Journal: J Immunol Methods Date: 1990-10-04 Impact factor: 2.303

3. Multiple fluorescence labeling with europium chelators. Application to time-resolved fluoroimmunoassays.

Authors: E P Diamandis; R C Morton; E Reichstein; M J Khosravi
Journal: Anal Chem Date: 1989-01-01 Impact factor: 6.986

Review 4. Immunoassays with time-resolved fluorescence spectroscopy: principles and applications.

Authors: E P Diamandis
Journal: Clin Biochem Date: 1988-06 Impact factor: 3.281

5. The biosynthesis of apo- and metalloalkaline phosphatases of Escherichia coli.

Authors: M I Harris; J E Coleman
Journal: J Biol Chem Date: 1968-10-10 Impact factor: 5.157

Review 6. Fluoroimmunoassay: present status and key problems.

Authors: E Soini; I Hemmilä
Journal: Clin Chem Date: 1979-03 Impact factor: 8.327

7. The synthesis and use of activated N-benzyl derivatives of diethylenetriaminetetraacetic acids: alternative reagents for labeling of antibodies with metal ions.

Authors: V M Mukkala; H Mikola; I Hemmilä
Journal: Anal Biochem Date: 1989-02-01 Impact factor: 3.365

Time-resolved fluoroimmunoassay.

1. A new fluorescence enhancement solution for europium-based time-resolved fluoroimmunoassays.

2. Time resolved fluoroimmunoassay of 7-methyl-2'-deoxyguanosine imidazole (ring open).

3. Multiple fluorescence labeling with europium chelators. Application to time-resolved fluoroimmunoassays.

Review 4. Immunoassays with time-resolved fluorescence spectroscopy: principles and applications.

5. The biosynthesis of apo- and metalloalkaline phosphatases of Escherichia coli.

Review 6. Fluoroimmunoassay: present status and key problems.

7. The synthesis and use of activated N-benzyl derivatives of diethylenetriaminetetraacetic acids: alternative reagents for labeling of antibodies with metal ions.

8. Europium as a label in time-resolved immunofluorometric assays.

9. A new europium chelate for protein labelling and time-resolved fluorometric applications.

10. Double-label time-resolved immunofluorometry of lutropin and follitropin in serum.

1. Cortisol and free thyroxine determination by time-resolved fluorometry in canine serum.