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Literature DB >> 10921947

Development of a quantitative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assays.

S D Pas¹, E Fries, R A De Man, A D Osterhaus, H G Niesters.

Abstract

A highly reproducible and sensitive real-time detection assay based on TaqMan technology was developed for the detection of hepatitis B virus (HBV) DNA and compared with two commercially available assays. The assay was validated with the Viral Quality Control panel, which also includes EUROHEP HBV DNA standards. This real-time PCR detection system had a dynamic range of 373 to 10(10) genome copies per ml and showed an excellent correlation with both the commercial HBV Digene Hybrid Capture II microplate assay (Digene Diagnostics) and the HBV MONITOR assay (Roche Diagnostics). To demonstrate its clinical utility, four chronically HBV-infected patients treated with lamuvidine were monitored using the three different assays. From the results we concluded that this assay is an excellent alternative for monitoring of HBV-infected patients in routine diagnostics and clinical practice, enabling the analysis of a large dynamic range of HBV DNA in a single, undiluted sample.

Entities: Chemical Disease Species

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Year: 2000 PMID： 10921947 PMCID： PMC87141

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

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Journal: J Hepatol Date: 1996-06 Impact factor: 25.083

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Authors: P Honkoop; R A de Man; H G Niesters
Journal: Ann Intern Med Date: 1998-04-15 Impact factor: 25.391

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Journal: Clin Diagn Virol Date: 1998-01

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Authors: H G Niesters; P Honkoop; E B Haagsma; R A de Man; S W Schalm; A D Osterhaus
Journal: J Infect Dis Date: 1998-05 Impact factor: 5.226

5. Lamivudine resistance in immunocompetent chronic hepatitis B. Incidence and patterns.

Authors: P Honkoop; H G Niesters; R A de Man; A D Osterhaus; S W Schalm
Journal: J Hepatol Date: 1997-06 Impact factor: 25.083

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Journal: J Clin Microbiol Date: 1995-01 Impact factor: 5.948

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Authors: V C Lai; R Guan; M L Wood; S K Lo; M F Yuen; C L Lai
Journal: J Clin Microbiol Date: 1999-01 Impact factor: 5.948

8. Quantitative detection of hepatitis B virus DNA in two international reference plasma preparations. Eurohep Pathobiology Group.

Authors: K H Heermann; W H Gerlich; M Chudy; S Schaefer; R Thomssen
Journal: J Clin Microbiol Date: 1999-01 Impact factor: 5.948

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Authors: M Krajden; J Minor; L Cork; L Comanor
Journal: J Viral Hepat Date: 1998-11 Impact factor: 3.728

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Authors: H L Zaaijer; F ter Borg; H T Cuypers; M C Hermus; P N Lelie
Journal: J Clin Microbiol Date: 1994-09 Impact factor: 5.948

43 in total

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Authors: J D Callahan; S J Wu; A Dion-Schultz; B E Mangold; L F Peruski; D M Watts; K R Porter; G R Murphy; W Suharyono; C C King; C G Hayes; J J Temenak
Journal: J Clin Microbiol Date: 2001-11 Impact factor: 5.948

2. Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds.

Authors: S Garcia; J M Crance; A Billecocq; A Peinnequin; A Jouan; M Bouloy; D Garin
Journal: J Clin Microbiol Date: 2001-12 Impact factor: 5.948

Review 3. Real-time PCR in virology.

Authors: Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal: Nucleic Acids Res Date: 2002-03-15 Impact factor: 16.971

4. Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA.

Authors: Rafael M Gordillo; Juan Gutiérrez; Manuel Casal
Journal: J Clin Microbiol Date: 2005-07 Impact factor: 5.948

5. Quantitative detection and rapid identification of human adenoviruses.

Authors: Rika Miura-Ochiai; Yasushi Shimada; Tsunetada Konno; Shudo Yamazaki; Koki Aoki; Shigeaki Ohno; Eitaro Suzuki; Hiroaki Ishiko
Journal: J Clin Microbiol Date: 2007-01-17 Impact factor: 5.948

6. Performance evaluation of TRUPCR^® HBV Real-time PCR assay for Hepatitis B virus DNA quantification in clinical samples: report from a tertiary care liver centre.

Authors: Sujata Lall; Manish C Choudhary; Supriya Mahajan; Guresh Kumar; Ekta Gupta
Journal: Virusdisease Date: 2019-01-05

7. Real Time-PCR HBV-DNA Analysis: Significance and First Experience in Armed Forces.

Authors: G S Chopra; P K Gupta; A C Anand; P P Varma; V Nair; Ramji Rai
Journal: Med J Armed Forces India Date: 2011-05-30

8. Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples.

Authors: Ming Shi; Yong Zhang; Ying-Hua Zhu; Jing Zhang; Wei-Jia Xu
Journal: World J Gastroenterol Date: 2008-01-21 Impact factor: 5.742

9. Comparison of reverse hybridization, microarray, and sequence analysis for genotyping hepatitis B virus.

Authors: Suzan D Pas; Nathalie Tran; Robert A de Man; C Burghoorn-Maas; Guy Vernet; Hubert G M Niesters
Journal: J Clin Microbiol Date: 2008-02-20 Impact factor: 5.948

10. Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA.

Authors: Dimitrios Paraskevis; Apostolos Beloukas; Catherine Haida; Antigoni Katsoulidou; Zisis Moschidis; Helen Hatzitheodorou; Agoritsa Varaklioti; Vana Sypsa; Angelos Hatzakis
Journal: Virol J Date: 2010-03-12 Impact factor: 4.099